Group II intron domain 5 facilitates a trans-splicing reaction.

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Population

Yeast mitochondrial DNA (aI5g) self-splicing group II intron in vitro model

Design

Preclinical

Authors

Discussion

May inform minimal RNA tools for splicing; leaves open translation to cardiovascular therapeutics.

Population

Yeast mitochondrial DNA (aI5g) self-splicing group II intron in vitro model

Intervention

Division within intron domain 4 and provision of domain 5 as a trans-acting RNA containing 42 nucleotides of intron sequence

Outcome

Trans-splicing reaction and cleavage at the 5' splice sitesurrogate

Intron domain 5 is essential for cleavage at the 5' splice site in group II introns and can facilitate trans-splicing in vitro.

Jarrell et al. (1988) studied this question.

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