What does this research mean for the field?

During the neuronal development of neural crest cells, the resting membrane potential is sequentially regulated by HERG-like and IRK-like currents, with HERG-like channels marking an immature and transient stage of differentiation. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

Investigate the profile and developmental transition of inward potassium currents in neural crest-derived neuronal precursors across progressive maturation stages.

December 1, 1997European Journal of Neuroscience

HERG‐ and IRK‐like Inward Rectifier Currents are Sequentially Expressed During Neuronal Development of Neural Crest Cells and their Derivatives

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Population

Quail neural crest cells and their derivatives from embryos

Design

Preclinical

Authors

AAAnnarosa ArcangeliElectrophysiology BRBarbara RosatiStony Brook School ACAlessia CherubiniUniversity of Florence

Discussion

Member takes

Implication

HERG-like currents mark transient immature stages in neural crest neurons; leaves open relevance to human cardiac or neural development.

Key Points

Investigate the profile and developmental transition of inward potassium currents in neural crest-derived neuronal precursors across progressive maturation stages.
Cultured quail neural crest cells in differentiative media and examined cells at early (12–24 h) versus late (>48 h) stages.
Harvested peripheral ganglionic derivatives (ciliary, dorsal root, and sympathetic ganglia, and adrenal medulla) from early and older (7–12 day) embryos.
Characterized biophysical profiles, pharmacological sensitivity to Cs+ and class III antiarrhythmics, and resting membrane potentials.
Immature neuronal precursors (12–24 h) expressed a HERG-like inward K+ current (qI[IR]) active at membrane potentials positive to -35 mV and blocked by Cs+ and class III antiarrhythmics.
Mature neurons (>48 h in culture or from 7–12 day embryos) switched expression from qI[IR] to a classical inward rectifier current (qI[IRK]) alongside fast Na+ currents.
The developmental transition from qI[IR] to qI[IRK] was accompanied across all neural crest derivatives by a resting membrane potential hyperpolarization of >20 mV.

Structured PICO

Population

Quail neural crest cells and their derivatives (ciliary ganglia, dorsal root ganglia, adrenal medulla and sympathetic chain ganglia) from embryos

Intervention

Culture in differentiative medium at various stages of maturation

Outcome

Inward K+ channel profile and resting membrane potential (VREST)surrogate

HERG-like channels mark an immature and transient stage of neuronal differentiation, sequentially replaced by IRK-like currents that hyperpolarize the resting potential.

Cite This Study

Arcangeli et al. (1997) studied this question.

synapsesocial.com/papers/6a1bf0a227b545b111a93afb https://doi.org/10.1111/j.1460-9568.1997.tb01689.x

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HERG‐ and IRK‐like Inward Rectifier Currents are Sequentially Expressed During Neuronal Development of Neural Crest Cells and their Derivatives

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

HERG‐ and IRK‐like Inward Rectifier Currents are Sequentially Expressed During Neuronal Development of Neural Crest Cells and their Derivatives

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider