Electrical properties associated with wide intercellular clefts in rabbit Purkinje fibres.
Population
Rabbit Purkinje fibres
Design
Preclinical
Authors
Discussion
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Implication
Rabbit Purkinje fibres may minimize non-uniformity artifacts in ionic studies; extends morphological data but leaves translation open.
Key Points
- To analyze the electrical properties and ionic mechanisms of rabbit Purkinje fibres with wide intercellular clefts.
- Micro-electrode recordings of electrical activity
- Two-micro-electrode voltage clamp experiments
- Exposure to K glutamate Tyrode solution during recovery
- Action potentials exhibit significant overshoot and plateau but lack pacemaker depolarization at negative potentials.
- Slow inward currents were observed that were resistant to tetrodotoxin and blocked by D600.
- Current changes due to potassium depletion were minimal compared to other cardiac preparations.
Structured PICO
P
Population
Rabbit Purkinje fibres
I
Intervention
Micro-electrode recordings of electrical activity and two-micro-electrode voltage clamp
O
Outcome
Passive electrical properties and ionic currents (slow inward current, transient outward current, late outward current)surrogate
Rabbit Purkinje fibres offer structural advantages for studying ionic permeability mechanisms with minimal interference from radial non-uniformities in membrane potential or ion concentration.
Cite This Study
Colatsky et al. (1979) studied this question.