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December 1, 1990Proceedings of the National Academy of SciencesOpen Access

Eukaryotic initiation factor 3 is required for poliovirus 2A protease-induced cleavage of the p220 component of eukaryotic initiation factor 4F.

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Population

HeLa cell extracts, E. coli expressing poliovirus 2A protease, and purified eukaryotic initiation factor 3…

Comparison

Addition of purified eIF-3 to an in vitro… vs Reaction mixtures without added eIF-3 or with…

Design

Preclinical

Authors

EWElizabeth E. WyckoffTroy UniversityJHJohn W.B. HersheyRockefeller FoundationEEE EhrenfeldUniversity of Utah

Discussion

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Implication

Supports eIF-3 as potential antiviral target; leaves open in vivo validation and clinical translation.

Structured PICO

P
Population
HeLa cell extracts, E. coli expressing poliovirus 2A protease (2Apro), and purified eukaryotic initiation factor 3 (eIF-3) from HeLa cells and rabbit reticulocytes
I
Intervention
Addition of purified eIF-3 to an in vitro reaction mixture containing p220 substrate and poliovirus 2Apro
C
Comparator
Reaction mixtures without added eIF-3 or with control E. coli extracts lacking 2Apro
O
Outcome
Cleavage of the p220 component of eukaryotic initiation factor 4Fsurrogate

Eukaryotic initiation factor 3 is required for the poliovirus 2A protease-induced cleavage of p220, a critical step in the viral shut-off of host cell protein synthesis.

Limitations

  • The exact role of eIF-3 in the cleavage reaction is unknown
  • The cellular protein that functions as the actual protease cleaving p220 has not been definitively identified

Cite This Study

Wyckoff et al. (1990) studied this question.

synapsesocial.com/papers/6a1bfd9426cb5670aa9d3847https://doi.org/10.1073/pnas.87.24.9529
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Inhibition of HeLa cell protein synthesis following poliovirus infection correlates with the proteolysis of a 220,000-dalton polypeptide associated with eucaryotic initiation factor 3 and a cap binding protein complex.1982 · 592 citations
  2. 2Poliovirus protease 3C (P3-7c) does not cleave P220 of the eucaryotic mRNA cap-binding protein complex1985 · 28 citations
  3. 3Association of cap-binding protein with eucaryotic initiation factor 3 in initiation factor preparations from uninfected and poliovirus-infected HeLa cells1982 · 50 citations
  4. 4Poliovirus translation: A paradigm for a novel initiation mechanism1989 · 44 citations
  5. 5Poliovirus mutant that does not selectively inhibit host cell protein synthesis.1985 · 160 citations