What does this research mean for the field?

Overexpression of the apolipoprotein CIII gene directly causes hypertriglyceridemia in vivo in a dose-dependent manner, identifying it as a potential primary etiology for the disorder in humans. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To determine whether overexpression of the human apolipoprotein CIII gene can act as a primary cause of hypertriglyceridemia in vivo.

August 17, 1990Science

Hypertriglyceridemia as a Result of Human apo CII Gene Expression in Transgenic Mice

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Why the study?

Does overexpression of the human apolipoprotein CIII gene cause hypertriglyceridemia in transgenic mice?

Population

Transgenic mice

Design

Preclinical

Authors

YIYasushi ItoJapan Aerospace Exploration Agency NANeal AzrolanMerck & Co., Inc., Rahway, NJ, USA (United States)AOAnita M. O'ConnellThe Heart Research Institute

Discussion

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Implication

Findings in transgenic mice do not alter clinical practice; leaves open apoCIII overexpression as a potential human hypertriglyceridemia etiology.

Key Points

To determine whether overexpression of the human apolipoprotein CIII gene can act as a primary cause of hypertriglyceridemia in vivo.
Generated two distinct transgenic mouse lines engineered to express the human apolipoprotein CIII gene.
Evaluated protein expression and triglyceride levels between a mouse line carrying 100 gene copies and a line carrying one to two gene copies.
Mice carrying 100 copies of the gene expressed high amounts of human apolipoprotein CIII and developed severe hypertriglyceridemia.
Mice carrying one to two copies expressed low amounts of the protein but still manifested mild hypertriglyceridemia.

Structured PICO

Does overexpression of the human apolipoprotein CIII gene cause hypertriglyceridemia in transgenic mice?

Population

Transgenic mice

Intervention

Expression of human apolipoprotein CIII gene (100 copies or 1-2 copies)

Outcome

Hypertriglyceridemiasurrogate

Overexpression of apolipoprotein CIII in transgenic mice causes hypertriglyceridemia, suggesting a possible etiology for the disorder in humans.

Cite This Study

Ito et al. (1990) studied this question.

synapsesocial.com/papers/6a1c0c08d54006be995f74ea https://doi.org/10.1126/science.2167514

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Implication

Findings in transgenic mice do not alter clinical practice; leaves open apoCIII overexpression as a potential human hypertriglyceridemia etiology.

Hypertriglyceridemia as a Result of Human apo CII Gene Expression in Transgenic Mice

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Hypertriglyceridemia as a Result of Human apo CII Gene Expression in Transgenic Mice

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider