In intact skeletal muscle, cAMP-dependent phosphorylation does not modulate acetylcholine receptor desensitization, indicating that forskolin's effects on desensitization are independent of cAMP.
Challenges prior cAMP-AChR desensitization links from biochemical studies; hypothesis-generating for forskolin's independent actions in muscle.
Biochemical and electrophysiological studies suggest that adenosine 3',5'-monophosphate (cAMP)-dependent phosphorylation of the nicotinic acetylcholine receptor channel is functionally significant because it modifies the receptor's rate of desensitization to acetylcholine. In studies that support this conclusion researchers have used forskolin to stimulate cAMP-dependent phosphorylation in intact muscle. It is now shown that although forskolin facilitated desensitization in voltage-clamped rat muscle, this effect was not correlated with the abilities of forskolin and forskolin analogs to activate adenylate cyclase or phosphorylate the receptor. Furthermore, elevation of intracellular cAMP or addition of the catalytic subunit of A-kinase failed to alter desensitization. Therefore, in intact skeletal muscle, cAMP-dependent phosphorylation does not modulate desensitization.
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Wagoner et al. (1988) studied this question.
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