What question did this study set out to answer?

To purify and characterize the structure, isoforms, and functional binding domains of the large myosin-binding subunit of smooth muscle myosin phosphatase.

December 1, 1994Journal of Biological ChemistryOpen Access

Characterization of the myosin-binding subunit of smooth muscle myosin phosphatase.

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Authors

HSH. ShimizuPolytechnic University MIMasaaki ItoNihon University MMMasaya MiyaharaHigh Energy Accelerator Research Organization

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Implication

Biochemical analysis uncovers the primary structure and binding domains of myosin phosphatase in avian smooth muscle, highlighting its role in muscle regulation.

Key Points

To purify and characterize the structure, isoforms, and functional binding domains of the large myosin-binding subunit of smooth muscle myosin phosphatase.
Purified the trimeric myosin phosphatase holoenzyme consisting of 130-kDa, 38-kDa, and 20-kDa subunits from chicken gizzard smooth muscle.
Isolated and sequenced overlapping cDNA clones from chicken gizzard cDNA libraries to characterize the full-length open reading frames and domain features.
Identified two isoforms of the large subunit with open reading frames of 2,889 and 3,012 bases encoding proteins of 963 and 1,004 amino acids (106,700 and 111,600 Da).
Demonstrated that the N-terminal third contains eight ankyrin-like repeats, with a 58,000-Da N-terminal fragment mediating binding to both myosin and the 38,000-Da catalytic subunit.

Cite This Study

Shimizu et al. (1994) studied this question.

synapsesocial.com/papers/6a1e40bf7f6e8bbb2368aff2 https://doi.org/10.1016/s0021-9258(18)43828-8

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Implication

Biochemical analysis uncovers the primary structure and binding domains of myosin phosphatase in avian smooth muscle, highlighting its role in muscle regulation.

Characterization of the myosin-binding subunit of smooth muscle myosin phosphatase.

Discussion

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Implication

Key Points

Cite This Study

Characterization of the myosin-binding subunit of smooth muscle myosin phosphatase.

Discussion

Member takes

Implication

Key Points

Cite This Study

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