What question did this study set out to answer?

To determine whether transforming growth factor-beta 1 modulates endothelial cell nitric oxide synthase (ecNOS) expression and activity in bovine aortic endothelial cells.

August 1, 1995Arteriosclerosis Thrombosis and Vascular Biology

Molecular Regulation of the Bovine Endothelial Cell Nitric Oxide Synthase by Transforming Growth Factor–β 1

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Authors

NINobutaka InoueKobe Kaisei Hospital RVRichard C. VenemaAugusta University HSHassan SayeghValve (United States)

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Implication

In vitro study reveals transforming growth factor-beta 1 stimulates endothelial nitric oxide synthase in bovine cells, highlighting promoter-mediated control via a nuclear factor-1 binding site.

Key Points

To determine whether transforming growth factor-beta 1 modulates endothelial cell nitric oxide synthase (ecNOS) expression and activity in bovine aortic endothelial cells.
Cultured bovine aortic endothelial cells were treated with transforming growth factor-beta 1 to quantify ecNOS mRNA, protein content, and enzymatic conversion of arginine to citrulline.
Transcriptional regulation was analyzed using promoter-luciferase reporter constructs, 5'-flanking deletion assays, gel mobility shift assays, and site-directed mutagenesis of a putative nuclear factor-1 site.
Transforming growth factor-beta 1 caused a dose-dependent increase in ecNOS mRNA and protein levels, concurrently raising nitric oxide synthase enzymatic activity and nitrogen oxide production.
Promoter deletion and gel shift assays localized the response element to nucleotides -1269 to -935 containing an active nuclear factor-1 binding sequence, and mutating this site significantly reduced promoter responsiveness.

Cite This Study

Inoue et al. (1995) studied this question.

synapsesocial.com/papers/6a1eaa13bf2a5d44faaf27df https://doi.org/10.1161/01.atv.15.8.1255

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Implication

In vitro study reveals transforming growth factor-beta 1 stimulates endothelial nitric oxide synthase in bovine cells, highlighting promoter-mediated control via a nuclear factor-1 binding site.

Molecular Regulation of the Bovine Endothelial Cell Nitric Oxide Synthase by Transforming Growth Factor–β 1

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Implication

Key Points

Cite This Study

Molecular Regulation of the Bovine Endothelial Cell Nitric Oxide Synthase by Transforming Growth Factor–β 1

Discussion

Member takes

Implication

Key Points

Cite This Study

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