Key result
Hepatic and extrahepatic angiotensinogen mRNA levels remained unaltered during the development of acute nephrotic syndrome induced by puromycin amino-nucleoside in rats.
Why the study?
Does acute nephrotic syndrome induced by PAN alter hepatic and extrahepatic angiotensinogen gene expression in rats?
Does acute nephrotic syndrome induced by PAN alter hepatic and extrahepatic angiotensinogen gene expression in rats?
Acute nephrotic syndrome induced by PAN does not alter hepatic and extrahepatic angiotensinogen mRNA levels in rats, despite changes in plasma and urine Ao levels.
Answers are grounded in this paper and cite their sources.
No basis to alter RAS therapies in nephrotic syndrome; leaves open post-transcriptional mechanisms in humans.
Plasma concentration and urine excretion of the renin-angiotensin system proteins are altered in rats with nephrotic syndrome (NS). In this work the messenger ribonucleic acid (mRNA) levels of angiotensinogen (Ao) were analyzed with the slot-blot hybridization technique in liver and other extrahepatic tissues: kidney, heart, brain, and adrenal gland from control, nephrotic, and pair-fed (PF) rats. NS was induced by a single injection of puromycin amino-nucleoside (PAN). Although a great urinary excretion and half-normal plasma levels of Ao were observed on day 6 after PAN injection, when NS was clearly established, hepatic Ao mRNA levels did not change. Furthermore, the Ao mRNA levels did not change in any of the extrahepatic tissues studied on day 6, nor did its hepatic levels at days 1, 3, 5, or 7 after PAN injection. These data suggest that the hepatic and extrahepatic Ao mRNA levels are unaltered during the development of the acute NS induced by PAN.
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Ibarra‐Rubio et al. (1997) studied Acute nephrotic syndrome. Puromycin amino-nucleoside (PAN) injection vs. Control and pair-fed (PF) rats was evaluated on Angiotensinogen (Ao) mRNA levels in hepatic and extrahepatic tissues. Hepatic and extrahepatic angiotensinogen mRNA levels remained unaltered during the development of acute nephrotic syndrome induced by puromycin amino-nucleoside in rats.
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