What is the clinical evidence from this study?

Study design: Other. Population: Angiotensin II-Dependent Hypertension. Intervention: Angiotensin II vs. Sham operation. Primary outcome: Renal and hepatic expression of angiotensinogen mRNA and protein levels.

What does this research mean for the field?

Chronic Angiotensin II infusion significantly increases intrarenal angiotensinogen mRNA levels and renal AngII contents, contributing to the sustained renal AngII-generating capacity observed in AngII-dependent hypertension. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To investigate whether chronic angiotensin II infusion increases renal angiotensinogen mRNA and protein levels.

March 1, 2001Journal of the American Society of NephrologyOpen Access

Expression of Angiotensinogen mRNA and Protein in Angiotensin II-Dependent Hypertension

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Key result

Chronic Angiotensin II infusion in rats significantly increased intrarenal angiotensinogen mRNA levels (ratio 0.82 vs 0.58) and renal AngII contents (273 vs 99 fmol/g) compared to sham.

Why the study?

Does chronic Angiotensin II infusion increase renal angiotensinogen mRNA and protein levels in Sprague-Dawley rats?

Population

Sprague-Dawley rats

Comparison

Angiotensin II infused subcutaneously for 13… vs Sham operations

Design

Preclinical

Follow-up

13 days

Authors

HKHiroyuki KoboriGeneral / Preventive / Lipids LHLisa M. Harrison‐BernardLouisiana State University Health Sciences Center New Orleans LNL. Gabriel NavarVascular Medicine

Discussion

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Implication

Hypothesis-generating in AngII rat models; leaves open clinical relevance for human hypertension or CKD.

Key Points

To investigate whether chronic angiotensin II infusion increases renal angiotensinogen mRNA and protein levels.
AngII was infused subcutaneously for 13 days into Sprague-Dawley rats using osmotic minipumps.
Control rats underwent sham operations.
mRNA and protein expression were analyzed using reverse transcription-PCR and Western blot techniques.
Systolic arterial BP increased to 184 +/- 3 mmHg in AngII-treated rats versus 125 +/- 1 mmHg in control rats.
Plasma renin activity decreased significantly in AngII-treated rats (0.2 +/- 0.1 ng AngI/ml per h).
Renal angiotensinogen mRNA levels and hepatic expression of the 64-kD protein increased significantly in AngII-treated rats compared to controls.

Structured PICO

Does chronic Angiotensin II infusion increase renal angiotensinogen mRNA and protein levels in Sprague-Dawley rats?

Population

Sprague-Dawley rats

Intervention

Angiotensin II (80 ng/min) infused subcutaneously for 13 days using osmotic minipumps

Comparator

Sham operations

Outcome

Renal and hepatic expression of angiotensinogen mRNA and protein levelssurrogate

Chronic Angiotensin II infusion increases intrarenal angiotensinogen mRNA levels, contributing to sustained renal AngII-generating capacity in hypertensive rats.

Cite This Study

Kobori et al. (2001) studied Angiotensin II-Dependent Hypertension. Angiotensin II vs. Sham operation was evaluated on Renal and hepatic expression of angiotensinogen mRNA and protein levels. Chronic Angiotensin II infusion in rats significantly increased intrarenal angiotensinogen mRNA levels (ratio 0.82 vs 0.58) and renal AngII contents (273 vs 99 fmol/g) compared to sham.

synapsesocial.com/papers/6a1d55597328fa9a742f57ac https://doi.org/10.1681/asn.v123431

Topics

Hypertension management

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Expression of Angiotensinogen mRNA and Protein in Angiotensin II-Dependent Hypertension

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Topics

Expression of Angiotensinogen mRNA and Protein in Angiotensin II-Dependent Hypertension

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Topics

Also Consider