Intracellular Ionic Remodeling During Fetal Development of Hereditary Cardiomyopathy of the Hamster

Figures

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  Figure 1. Examples of a typical Ca2+ fluorescence intensity of top view image quantitative 3D top-view image of cardiomyocytes from normal from 15-day-old fetal normal hamster (NH) and HCMH. The pseudo color scale represents NHE1 fluorescence intensity ranging from 0 (no fluorescence, black color) to 255 (maximum fluorescence, white color). The white scale bar is μm. The green color of the nucleus (Syto11) has no measurable meaning.

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  Figure 2. Resting whole-cell, cytosolic, and nuclear calcium levels in cardiomyocytes of 15 days old of fetal NH and HCMHs. Histograms showing the whole-cell (A), cytosolic (B), and nuclear (C) resting calcium levels of 15-day-old fetal cardiomyocytes of NH and HCMH. The values are represented as mean ± standard error of the mean. * p < 0.05, and **** p < 0.0001. Calcium’s relative concentration is expressed in μm3.

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  Figure 3. Resting whole-cell, cytosolic, and nuclear sodium levels in cardiomyocytes of 15 days old of fetal NH and HCMHs. Histograms showing the whole-cell (A), cytosolic (B), and nuclear (C) resting sodium levels of 15-day-old fetal cardiomyocytes of NH and HCMH. The values are represented as mean ± standard error of the mean. * p < 0.05. Sodium’s relative concentration is expressed in μm3.

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  Figure 4. Resting whole-cell, cytosolic, and nuclear ROS levels in cardiomyocytes of 15 days old of fetal NH and HCMHs. Histograms showing differences in the resting levels of intracellular ROS between the whole-cell, cytosolic, and nuclear compartments of NH and HCMH cardiomyocytes. The values are represented as mean ± standard error of the mean. * p < 0.05. ROS’ relative concentration is expressed in μm3.

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  Figure 5. Examples of a typical NHE1 fluorescence intensity and distribution Fishnet Lookthrough quantitative 3D image of cardiomyocytes from normal from 15-day-old fetal normal hamster (NH) and HCMH. The pseudocolor scale represents NHE1 fluorescence intensity ranging from 0 (no fluorescence, black color) to 255 (maximum fluorescence, white color). The white scale bar is μm. The green color of the nucleus (Syto11) has no measurable meaning.

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  Figure 6. Relative density of NHE-1 in the whole cell, nucleus, and cytosol in cardiomyocytes of 15 days old of fetal NH and HCMHs. Histograms showing an increase in the relative density of NHE-1 level in the whole cell, nucleus, and cytosol of cardiomyocytes of HCMH. The values are represented as mean ± standard error of the mean. * p < 0.05, ** p < 0.01. NHE-1 relative density is expressed in μm3.