June 1, 1990Circulation Research
Control of coronary vascular tone by nitric oxide.
Population
Isolated, constant-flow-perfused guinea pig hearts
Comparison
Infusion of nitric oxide, L-arginine… vs Basal/unstimulated state
Design
Preclinical
Authors
Discussion
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Implication
Basal NO may regulate coronary resistance tone; extends animal observations but leaves open human translation.
Key Points
- This research aims to explore the effects of nitric oxide on coronary vascular tone and its mechanisms of action.
- Used a specific difference-spectrophotometric method to measure nitric oxide release
- Analyzed the effects of various compounds on coronary resistance and cGMP release
- Conducted experiments on isolated, constant-flow-perfused guinea pig hearts
- Authentic NO decreased vascular resistance and enhanced cGMP release fivefold
- Bradykinin increased coronary vessel dilation associated with NO and cGMP release
- Oxyhemoglobin and methylene blue increased coronary resistance and reduced cGMP release by 31%
Structured PICO
P
Population
Isolated, constant-flow-perfused guinea pig hearts
I
Intervention
Infusion of nitric oxide (NO), L-arginine, NG-monomethyl L-arginine, bradykinin, acetylcholine, ATP, and serotonin
C
Comparator
Basal/unstimulated state
O
Outcome
Coronary vascular resistance and release of NO and cyclic GMP (cGMP)surrogate
Basal formation of nitric oxide plays an important role in setting the resting tone of coronary resistance vessels and mediates vasodilation induced by bradykinin, ATP, acetylcholine, and serotonin.
Cite This Study
Kelm et al. (1990) studied this question.