Key result
Bovine fetal aortic endothelial cells accumulate and release ACE into the culture medium, with 80-85% of total activity detected in the medium 21 days following subcultivation.
In vitro, bovine fetal aortic endothelial cells synthesize and release ACE into the culture medium, with medium-associated activity eventually comprising the majority of total ACE activity.
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Endothelial ACE production shown in vitro; leaves open relevance to human vascular disease and ACE inhibitor effects.
Bovine fetal aortic endothelial cells cultured in serum-containing medium accumulate angiotensin I-converting enzyme (ACE) activity and also release it into the culture medium. Following subcultivation of a confluent culture using trypsin-EDTA, cellular ACE activity falls 50% within 8 h, but no ACE activity is detected in the medium, suggesting intracellular loss of the enzyme activity. ACE activity reappears in both the cell lysate and culture medium after the culture becomes confluent. The rate of accumulation of ACE activity released into the medium is always greater than that for cellular activity. For example, 21 days following subcultivation 80-85% of the total culture activity is detected in the medium. Both cellular and medium-associated ACE decrease proportionately as the culture progresses through its in vitro lifespan.
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Noveral et al. (1987) studied this question. Subcultivation using trypsin-EDTA was evaluated on Angiotensin I-converting enzyme (ACE) activity in cell lysate and culture medium. Bovine fetal aortic endothelial cells accumulate and release ACE into the culture medium, with 80-85% of total activity detected in the medium 21 days following subcultivation.
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