Lipoprotein lipase and metabolic activities in incubated bovine adipose tissue explants: effects of insulin, dexamethasone, and fetal bovine serum

View Full Paper

Ask AI

Bookmark

Population

Perirenal adipose tissue explants from 9 nonlactating, nonpregnant Holstein cows

Comparison

Incubation with insulin, dexamethasone, and/or… vs Basal conditions or insulin-supplemented medium…

Design

Preclinical

Follow-up

24 or 48 hours

Authors

Discussion

Highlights insulin-dexamethasone synergy on LPL in bovine explants; extends mechanistic insights but leaves clinical relevance open.

Population

Perirenal adipose tissue explants from 9 nonlactating, nonpregnant Holstein cows (subjected to limited feed for 10 d then overfed for 3 to 5 wk)

Intervention

Incubation with insulin (2 mU/mL), dexamethasone (10 nM or 100 nM), and/or 10% fetal bovine serum

Comparator

Basal conditions (unsupplemented medium) or insulin-supplemented medium alone

Outcome

Lipoprotein lipase (LPL) activity, glucose and acetate utilization, and glucose-6-phosphate dehydrogenase (G6PDH) activitysurrogate

In bovine adipose tissue explants, insulin and dexamethasone interact to regulate lipoprotein lipase activity and lipogenic enzyme function.

Faulconnier et al. (1994) studied this question.

View Full Paper

Ask AI

Bookmark