January 1, 1987Nucleic Acids ResearchOpen Access

in vitromolecular genetics as a tool for determining the differential cleavage specificities of the polivirus 3C proteinase

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Population

In vitro system using a complete cDNA copy of the entire poliovirus genome inserted into a bacteriophage T7…

Design

Preclinical

Authors

MYM F Ypma-WongUniversity of California, Irvine BSBert L. SemlerUniversity of California, Irvine

Discussion

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Implication

Findings on poliovirus 3C protease requirements should not yet guide therapies; leaves open in vivo validation of P3 domain roles.

Structured PICO

Population

In vitro system using a complete cDNA copy of the entire poliovirus genome inserted into a bacteriophage T7 transcription vector

Intervention

Introduction of proteinase and/or cleavage site mutations into the cDNA, followed by in vitro transcription and translation

Outcome

Phenotypic expression of mutations, specifically cleavage specificities and bimolecular cleavage events of the 3C proteinasesurrogate

The study provides an in vitro system demonstrating that the poliovirus 3C proteinase requires different regions of the P3 precursor for specific cleavage events.

Cite This Study

Ypma-Wong et al. (1987) studied this question.

synapsesocial.com/papers/6a20b46bf778797513eb8a17 https://doi.org/10.1093/nar/15.5.2069

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in vitromolecular genetics as a tool for determining the differential cleavage specificities of the polivirus 3C proteinase

Discussion

Member takes

Implication

Structured PICO

Cite This Study

in vitromolecular genetics as a tool for determining the differential cleavage specificities of the polivirus 3C proteinase

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider