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December 1, 1985Journal of General Virology

Production of a Monoclonal Antibody against an Epitope on HeLa Cells that Is the Functional Poliovirus Binding Site

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Population

HeLa cells, cell lines of primate, pig, dog, or rodent origin, and 40 clones of human-mouse cell hybrids

Design

Preclinical

Authors

PNP. NobisLeibniz Institute of Virology (LIV)RZReinhard ZibirreUniversität HamburgGMG MeyerBeiersdorf (Germany)

Discussion

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Implication

Provides in vitro evidence for poliovirus receptor blockade; leaves open clinical translation pending in vivo validation.

Key Points

  • This research aimed to isolate and characterize a monoclonal antibody that binds to the poliovirus receptor on HeLa cells.
  • Isolated and characterized monoclonal antibody D171 that reacts with HeLa cell plasma membranes.
  • Assessed protective effects of D171 against cytopathic effects of poliovirus type 1.
  • Analyzed binding compatibility in human-mouse cell hybrids to correlate susceptibility to poliovirus.
  • D171 protected HeLa cells from poliovirus type 1 cytopathic effects with confirmed binding to all viral serotypes.
  • Specific binding of D171 was observed on human or primate cell lines, but not on cells from pig, dog, or rodents.
  • Binding sites for 125I-labelled D171 were effectively blocked after preincubation with poliovirus, indicating D171 recognizes the poliovirus receptor.

Structured PICO

P
Population
HeLa cells, cell lines of primate, pig, dog, or rodent origin, and 40 clones of human-mouse cell hybrids
I
Intervention
Monoclonal antibody D171 (immunoglobulin or Fab fragments)
O
Outcome
Protection against cytopathic effect of poliovirus and specific binding to the poliovirus receptor sitesurrogate

The monoclonal antibody D171 specifically recognizes and blocks the functional poliovirus binding site on susceptible human and primate cells.

Cite This Study

Nobis et al. (1985) studied this question.

synapsesocial.com/papers/6a20b46bf778797513eb8a1bhttps://doi.org/10.1099/0022-1317-66-12-2563
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Also Consider

Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Monoclonal antibodies that inhibit attachment of group B coxsackieviruses1983 · 22 citations
  2. 2Protein Kinase Activity in Purified Poliovirus Particles and Empty Viral Capsid Preparations1984 · 10 citations