A HeLa-derived cell line (PRHC-1) was established that is persistently resistant to poliovirus infection due to a lack of binding activity, without significant chromosomal differences from parental cells.
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PRHC-1 provides a stable poliovirus-resistant HeLa model; leaves open its utility for mechanistic virology studies.
A cell line from HeLa cells was established, which did not show a cytopathogenic effect (CPE) to poliovirus infection. The cell line, designated PRHC-1, did not regain susceptibility after more than 100 passages for one year. The poliovirus type 1-Mahoney, Sabin -2 and Sabin -3 also were not infective to PRHC -1 cells, however a bovine enterovirus (MZ-468) was. No binding activity of PRHC -1 cells to poliovirus Sabin -1 was observed. A chromosome analysis demonstrated no significant difference between PRHC -1 and its parental HeLa cells. Immunofluorescence staining demonstrated no persistent infection in PRHC -1 cells.
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Chinami et al. (1986) studied this question.
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