What is the clinical evidence from this study?

Study design: Other. Population: Aging in canine right atrial cells. Intervention: Aging (Aged cells) vs. Adult cells (2-5 years). Primary outcome: L-type Ca(2+) currents and potassium currents (Ito, Isus).

What question did this study set out to answer?

Examine the differences in ionic currents between adult and aged canine right atrial cells to understand changes in action potentials.

April 30, 2003Cardiovascular ResearchOpen Access

Calcium and potassium currents in cells from adult and aged canine right atria

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Key result

Aged canine right atrial cells exhibited a 47% reduction in peak L-type calcium current and significant increases in transient outward and sustained potassium currents compared to adult cells.

Population

Cells dispersed from normal adult (2-5 years) and older dogs (Aged, >8 years) right atria

Comparison

Whole cell patch clamp recording of L-type Ca… vs Adult versus Aged canine right atrial cells

Design

Preclinical

Authors

WDWen DunColumbia University Irving Medical Center

Discussion

Member takes

Implication

May inform mechanisms of age-related canine atrial remodeling; leaves open human translation and clinical relevance.

Key Points

Examine the differences in ionic currents between adult and aged canine right atrial cells to understand changes in action potentials.
Used whole cell patch clamp techniques to measure L-type Ca(2+) and K(+) currents in cells from adult (2-5 years) and aged dogs (>8 years).
Evaluated currents under adrenergic stimulation conditions and measured the effects of tetraethylammonium chloride on potassium currents.
Aged cells exhibited a 47% reduction in peak L-type calcium current (I(CaL)) and a 43% reduction in barium current (I(BaL)).
Potassium currents were significantly increased in aged cells by 31% and 27% for transient outward (I(to)) and sustained currents (I(sus)) respectively.
TEA increased I(to) in aged cells, making them more sensitive to potassium current inhibition compared to adult cells.

Structured PICO

Population

Cells dispersed from normal adult (2-5 years) and older (>8 years) canine right atria.

Exposure

Whole cell patch clamp recording of L-type Ca(2+) currents and potassium currents (I(to) and I(sus)), including responses to isoproterenol and tetraethylammonium chloride (TEA)

Comparator

Adult (2-5 years) versus Aged (>8 years) canine right atrial cells

Outcome

L-type Ca(2+) currents (I(CaL)) and potassium currents (I(to) and I(sus))surrogate

Aged canine right atrial cells exhibit reduced calcium currents and augmented outward potassium currents, which may contribute to age-related changes in action potential contours.

Cite This Study

Wen Dun (2003) studied Aging in canine right atrial cells. Aging (Aged cells) vs. Adult cells (2-5 years) was evaluated on L-type Ca(2+) currents and potassium currents (Ito, Isus). Aged canine right atrial cells exhibited a 47% reduction in peak L-type calcium current and significant increases in transient outward and sustained potassium currents compared to adult cells.

synapsesocial.com/papers/6a20bb57e19207255aa0263a https://doi.org/10.1016/s0008-6363(03)00288-8

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Calcium and potassium currents in cells from adult and aged canine right atria

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Calcium and potassium currents in cells from adult and aged canine right atria

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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