What question did this study set out to answer?

The study investigates how aging affects K+ and Ca2+ currents in cardiac myocytes, assessing potential contributions to cardiac dysfunction.

September 1, 2000AJP Heart and Circulatory Physiology

Aging-associated changes in whole cell K⁺ and L-type Ca²⁺ currents in rat ventricular myocytes

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Population

Ventricular myocytes isolated from young adult (6 mo) and aged (>27 mo) Fischer 344 and Long-Evans rats

Comparison

Aging (>27 months old) vs Young adult (6 months old)

Design

Preclinical

Authors

SLShi J. LiuUniversity of Arkansas for Medical Sciences RWRichard P. WyethVirginia Tech RMRussell B. MelchertUniversity of Missouri–Kansas City

Discussion

Member takes

Implication

May underlie prolonged repolarization in aged rat myocytes; leaves open translation to human age-related arrhythmias.

Key Points

The study investigates how aging affects K+ and Ca2+ currents in cardiac myocytes, assessing potential contributions to cardiac dysfunction.
Used whole cell patch-clamp techniques on ventricular myocytes from young (6 mo) and aged (>27 mo) Fischer 344 rats.
Measured inward rectifier K(+) current (I(K1)), transient outward K(+) current (I(to)), and L-type Ca(2+) channel current (I(Ca,L)).
Compared findings with myocytes from Long-Evans rats to confirm observed patterns.
Aged myocytes showed same peak I(K1) and larger slope conductance but reduced steady-state I(K1).
Increased I(to) activation rate in aged myocytes, but inactivation kinetics and steady-state inactivation remained unaltered.
Peak I(Ca,L) reduced in aged myocytes with slower inactivation, indicating altered calcium handling.

Structured PICO

Population

Ventricular myocytes isolated from young adult (6 mo) and aged (>27 mo) Fischer 344 and Long-Evans rats

Intervention

Aging (>27 months old)

Comparator

Young adult (6 months old)

Outcome

Inward rectifier K(+) current (I(K1)), transient outward K(+) current (I(to)), L-type Ca(2+) channel current (I(Ca,L)), and action potential durationsurrogate

Aging alters key cardiac membrane currents and prolongs action potential duration in rat ventricular myocytes, potentially contributing to age-related cardiac dysfunction.

Cite This Study

Liu et al. (2000) studied this question.

synapsesocial.com/papers/6a6fe62235aa2c282ce13616 https://doi.org/10.1152/ajpheart.2000.279.3.h889

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Aging-associated changes in whole cell K⁺ and L-type Ca²⁺ currents in rat ventricular myocytes

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Aging-associated changes in whole cell K⁺ and L-type Ca²⁺ currents in rat ventricular myocytes

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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