Key result
Decreasing pH from 7.0 to 6.0 significantly reduced site II Ca(2+) affinity of cardiac troponin C, an effect that was modulated by isoform-specific interactions with troponin I and troponin T.
Population
Pyrene labelled cardiac troponin C in isolation and in complex with porcine cardiac or chicken pectoral…
Comparison
Increasing acidity and replacement of cardiac… vs pH 7.0 and cardiac troponin isoforms
Design
Preclinical
Authors
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May explain pH effects on myofilament Ca2+ sensitivity in ischemia; leaves open isoform roles in human troponin regulation.
Changes in troponin C conformation resulting from isoform-specific interactions with troponin I and T may play an integral role in the effect of pH on myofilament calcium sensitivity during ischemia.
Ying‐Ming Liou (2004) studied Acidosis in cardiac muscle (ischemia of heart failure). Increasing acidity (pH 6.0) vs. pH 7.0 was evaluated on Ca(2+) induced conformational changes and site II Ca(2+) affinity of PIA-cTnC. Decreasing pH from 7.0 to 6.0 significantly reduced site II Ca(2+) affinity of cardiac troponin C, an effect that was modulated by isoform-specific interactions with troponin I and troponin T.
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