Angiotensin II-induced myocardial fibrosis in rats: role of nitric oxide, prostaglandins and bradykinin

Male Sprague-Dawley rats

14-day infusion of Angiotensin II (150 ng/kg/min, s.c.) alone or co-administered with L-NAME (10 mg/kg/day in drinking water), indomethacin (2 mg/kg/day in drinking water), or Hoe140 (115 ng/kg/min, s.c.)

Untreated controls

Perivascular and interstitial fibrosis (measured as perivascular collagen volume fraction [PCVF] and interstitial collagen volume fraction [ICVF] via picrosirius-stained tissue)surrogate