Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
April 1, 1995Hypertension

Expression of Angiotensin-Converting Enzyme in Renovascular Hypertensive Rat Kidney

View Full Paper
Ask AI
Bookmark
Share

Why the study?

Does clipping of the left renal artery alter the gene expression of renin-angiotensin system components in the kidneys of Sprague-Dawley rats?

Population

Sprague-Dawley rats

Comparison

Clipping of the left renal artery vs Sham operation

Design

Preclinical

Follow-up

28 days

Authors

MMMonika MaiFriedrich-Alexander-Universität Erlangen-NürnbergKHKarl F. HilgersGeneral / Preventive / LipidsJWJürgen WagnerVogtland-Klinik

Discussion

Loading...

Member takes

Implication

Does not alter clinical management of renovascular hypertension; leaves open the role of ACE upregulation in human disease.

Key Points

  • The aim was to investigate the expression of renin and components of the renin-angiotensin system in response to renal artery stenosis in rats.
  • Two-kidney, one clip renovascular hypertension model was used in Sprague-Dawley rats.
  • Clipping of the left renal artery initiated hypertension; control rats underwent sham operation.
  • RNA from the kidneys was analyzed using Northern blotting and densitometry to measure gene expression.
  • Renin expression increased by 149% in the clipped kidney on day 2 and decreased by 82% in the nonclipped kidney compared to controls.
  • Angiotensin-converting enzyme expression rose by 158% post-clipping and remained elevated (66% on day 28) in the clipped kidneys.
  • Angiotensin II type 1 receptor expression decreased in nonclipped kidneys, with minimal change in clipped ones.

Structured PICO

Does clipping of the left renal artery alter the gene expression of renin-angiotensin system components in the kidneys of Sprague-Dawley rats?

P
Population
Sprague-Dawley rats
I
Intervention
Clipping of the left renal artery (two-kidney, one clip renovascular hypertension model)
C
Comparator
Sham operation
O
Outcome
Gene expression of angiotensinogen, angiotensin-converting enzyme, angiotensin II type 1 receptor, and renin in renal cortical RNAsurrogate

In a rat model of renovascular hypertension, components of the renin-angiotensin system, including ACE, are differentially expressed in clipped kidneys.

Cite This Study

Mai et al. (1995) studied this question.

synapsesocial.com/papers/6a214812c4e60624665adec8https://doi.org/10.1161/01.hyp.25.4.674
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Interrelation between renin mRNA levels, renin secretion, and blood pressure in two-kidney, one clip rats.1994 · 27 citations
  2. 2Renin, Angiotensinogen, and Kallikrein Gene Expression in Two-Kidney Goldblatt Hypertensive Rats1993 · 50 citations
  3. 3Decreased perfusion pressure modulates renin and ANG II type 1 receptor gene expression in the rat kidney1993 · 44 citations
  4. 4Regional angiotensin II production in essential hypertension and renal artery stenosis.1993 · 104 citations
  5. 5Molecular cloning of rat renin cDNA and its gene.1987 · 164 citations