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January 1, 1988The Journal of General PhysiologyOpen Access

Voltage-dependent K+ currents and underlying single K+ channels in pheochromocytoma cells.

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Key result

Patch-clamp recordings in PC-12 cells revealed at least four distinct types of depolarization-activated K+ channels (Kw, Kz, Ky, Kx) with typical chord conductances of 18, 12, 7, and 7 pS.

Population

Clonal pheochromocytoma (PC-12) cells

Design

Preclinical

Authors

THToshinori HoshiLeibniz University HannoverRARichard W. AldrichElectrophysiology

Discussion

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Implication

Characterizes four K+ channels accounting for PC-12 currents; leaves open their molecular identities and roles in neuronal physiology.

Key Points

  • This research aims to identify and characterize K+ currents and channel types in pheochromocytoma cells.
  • Studied whole-cell K+ currents using patch-clamp technique.
  • Examined activation and inactivation properties of K+ channels in clonal PC-12 cells.
  • Tested responses to pharmacological agents and voltage-clamp pulse protocols.
  • Identified four distinct types of K+ channels (Kw, Kz, Ky, Kx) with varying conductance (7-18 pS).
  • All four K+ channels activated with low internal Ca++ concentrations (1 nM).
  • Channel properties correlated with whole-cell K+ current characteristics.

Structured PICO

P
Population
In vitro study of clonal pheochromocytoma (PC-12) cells to characterize voltage-dependent K+ currents and single K+ channels.
I
Intervention
Patch-clamp recording technique with depolarizing pulses and external application of tetraethylammonium+, 4-aminopyridine, and quinidine
O
Outcome
Properties of whole-cell K+ currents and voltage-dependent activation and inactivation properties of single K+ channelssurrogate

PC-12 cells possess at least four distinct types of depolarization-activated K+ channels that account for their whole-cell K+ current properties.

Cite This Study

Hoshi et al. (1988) studied Pheochromocytoma. Depolarizing pulses and pharmacological agents was evaluated on Properties of whole-cell K+ currents and voltage-dependent activation/inactivation of single K+ channels. Patch-clamp recordings in PC-12 cells revealed at least four distinct types of depolarization-activated K+ channels (Kw, Kz, Ky, Kx) with typical chord conductances of 18, 12, 7, and 7 pS.

synapsesocial.com/papers/6a21c73f7cd64f3298c00a86https://doi.org/10.1085/jgp.91.1.73
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Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Properties of single calcium‐activated potassium channels in cultured rat muscle1982 · 715 citations
  2. 2Development of a delayed outward-rectifying K+ conductance in cultured mouse peritoneal macrophages.1984 · 132 citations
  3. 3Three kinetically distinct potassium channels in mouse neuroblastoma cells.1988 · 51 citations