Key result
Activated Gs alpha, PKA, and okadaic acid increased initial L-type Ca channel activity and delayed rundown in planar lipid bilayers, while activated Gi1 alpha inhibited Gs alpha stimulation.
Population
L-type Ca channels incorporated from porcine ventricular sarcolemma into planar lipid bilayers
Comparison
Activated G proteins, adenosine 3',5'-cyclic… vs Unactivated Gs alpha, heat-denatured activated…
Design
Preclinical
Authors
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Hypothesis-generating for G-protein/PKA regulation of cardiac L-type Ca channels; extends bilayer data but leaves open in vivo translation.
Activated Gs alpha and PKA independently stimulate cardiac L-type Ca channels and delay their rundown in planar lipid bilayers.
Wang et al. (1993) studied this question. Activated G proteins (Gs alpha and Gi1 alpha), PKA, and okadaic acid vs. Unactivated Gs alpha, heat-denatured Gs alpha, or baseline was evaluated on Channel activity and rundown. Activated Gs alpha, PKA, and okadaic acid increased initial L-type Ca channel activity and delayed rundown in planar lipid bilayers, while activated Gi1 alpha inhibited Gs alpha stimulation.
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