During CABG, IL-6 and E-selectin concentrations were significantly higher in the coronary sinus than in arterial blood, indicating the myocardium is a predominant source of these mediators.
Observational (n=12)
Does the heart or lungs produce proinflammatory cytokines following cardiopulmonary bypass in patients undergoing CABG?
The myocardium, rather than the lungs, is a predominant source of IL-6 and E-selectin release following cardiopulmonary bypass, suggesting myocardial ischemia is crucial in the pathogenesis of the inflammatory response.
OBJECTIVE: Proinflammatory cytokines, such as interleukin-6 (IL-6), and soluble adhesion molecules, such as E-selectin, may play an important role in patient response to cardiopulmonary bypass (CPB). We sought to define whether the heart and the lungs serve as important sources of these inflammatory mediators under clinical conditions of myocardial revascularization using CPB and cardioplegic arrest. METHODS: Plasma levels of IL-6 and E-selectin were measured in coronary sinus (CS), arterial, pulmonary arterial (PA) and left atrial (LA) blood samples taken from 12 consecutive patients (68.3 +/- 11 years; five females) undergoing coronary artery bypass grafting (CABG). Blood samples were collected preoperatively, after reperfusion, and 1, 6, 12 and 18 h following surgery. CS and LA blood was drawn using transcutaneous catheters. PA artery blood was obtained through a Swan-Ganz catheter. Cytokine levels were determined by standard enzyme linked immunosorbent assay (ELISA) technique. RESULTS: A mean of 3.8 +/- 1 coronary anastomoses were performed. The CPB time and aortic X-clamp time were 91 +/- 15 and 45 +/- 10 min, respectively. IL-6 levels increased significantly after CPB and peaked 6 h postoperatively. There was also a significant increase of E-selectin levels with an onset at 1 h and a peak at 12 h postoperatively. At all time points the IL-6 and E-selectin concentrations were significantly higher in the CS than in arterial blood. In contrast, the levels of both mediators measured in the LA were significantly lower than those in the PA. CONCLUSION: The reperfusion of ischemic myocardium during CABG results in a significant increase in plasma levels of IL-6 and E-selectin. Our data indicate that the myocardium, but not the lungs, is a predominant source of IL-6 and E-selectin release following CPB. The lungs may consume rather than release those mediators during reperfusion. Not the CPB per se, but the myocardial ischemia seems to be crucial in the pathogenesis of the inflammatory response observed following open heart surgery.
Liebold et al. (Mon,) conducted a observational in Coronary artery bypass grafting (CABG) (n=12). Cardiopulmonary bypass (CPB) and cardioplegic arrest was evaluated on Plasma levels of IL-6 and E-selectin in coronary sinus, arterial, pulmonary arterial, and left atrial blood. During CABG, IL-6 and E-selectin concentrations were significantly higher in the coronary sinus than in arterial blood, indicating the myocardium is a predominant source of these mediators.