Measurement of Na(+)-K+ pump current in isolated rabbit ventricular myocytes using the whole-cell voltage-clamp technique. Inhibition of the pump by oxidant stress.
Key result
A 10-minute exposure to oxidant stress generated by photoactivated rose bengal reduced the Na(+)-K+ pump current in isolated rabbit ventricular myocytes by approximately 50% at 0 mV.
Population
Isolated rabbit ventricular myocytes
Comparison
Oxidant stress induced by photoactivation of… vs Baseline/absence of oxidant stress
Design
Preclinical
Follow-up
10 minutes
Authors
Discussion
Loading...
Member takes
Implication
Oxidant stress may contribute to Na/K pump inhibition during ischemia/reperfusion; this Level 5 animal finding leaves open its role in human calcium overload.
Structured PICO
P
Population
Isolated rabbit ventricular myocytes
I
Intervention
Oxidant stress induced by photoactivation of rose bengal (50 nM)
C
Comparator
Baseline/absence of oxidant stress
O
Outcome
Na(+)-K+ pump currentsurrogate
Oxidant stress significantly inhibits the Na(+)-K+ pump current in ventricular myocytes without causing nonspecific membrane damage, potentially contributing to ischemia/reperfusion injury and calcium overload.
Cite This Study
Shattock et al. (1993) studied this question. Oxidant stress (photoactivation of rose bengal) vs. Baseline/control conditions was evaluated on Na(+)-K+ pump current measured at 0 mV. A 10-minute exposure to oxidant stress generated by photoactivated rose bengal reduced the Na(+)-K+ pump current in isolated rabbit ventricular myocytes by approximately 50% at 0 mV.