What question did this study set out to answer?

This research aims to explore how reactive oxygen intermediates induce arrhythmias in isolated rabbit ventricular cells.

February 1, 1991Circulation ResearchOpen Access

Membrane potential fluctuations and transient inward currents induced by reactive oxygen intermediates in isolated rabbit ventricular cells.

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Why the study?

Does exposure to reactive oxygen intermediates induce arrhythmogenic membrane potential fluctuations and transient inward currents in isolated rabbit ventricular cells?

Key Points

This research aims to explore how reactive oxygen intermediates induce arrhythmias in isolated rabbit ventricular cells.
Isolated rabbit ventricular cells were investigated using whole-cell voltage- and current-clamp techniques.
Singlet oxygen and superoxide were generated by photoactivation of rose bengal at concentrations of 10-100 nM.
Voltage and transient inward currents were measured on repolarization after depolarizing clamp steps.
Spontaneous membrane potential fluctuations measured at plateau potentials were 2-5 mV with a frequency of 2.6 Hz, and at resting membrane potential were 1-3 mV with a frequency of 1.5 Hz.
Transient inward currents peaked at -20 mV and -70 mV at approximately 3.7 Hz and 2.3 Hz, respectively.
Electrogenic sodium-calcium exchange was identified as the primary contributor to inward currents, reducing by about 90% when sodium was replaced with lithium.

Structured PICO

Does exposure to reactive oxygen intermediates induce arrhythmogenic membrane potential fluctuations and transient inward currents in isolated rabbit ventricular cells?

Population

Isolated rabbit ventricular cells

Intervention

Exposure to singlet oxygen and superoxide generated by the photoactivation of rose bengal (10-100 nM)

Outcome

Membrane potential fluctuations and transient inward currents measured via whole-cell voltage- and current-clamp techniquessurrogate

Oxidant stress induces arrhythmogenic membrane potential fluctuations and transient inward currents in ventricular cells, primarily driven by electrogenic Na-Ca exchange, providing a potential mechanism for reperfusion-induced arrhythmias.

Discussion

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Implication

May link oxidant stress to reperfusion arrhythmias via Na-Ca exchange; hypothesis-generating and requires in vivo confirmation before clinical relevance.

Cite This Study

Matsuura et al. (1991) studied this question.

synapsesocial.com/papers/6a227fe8ffccceb004b73248 https://doi.org/10.1161/01.res.68.2.319

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Implication

May link oxidant stress to reperfusion arrhythmias via Na-Ca exchange; hypothesis-generating and requires in vivo confirmation before clinical relevance.

Membrane potential fluctuations and transient inward currents induced by reactive oxygen intermediates in isolated rabbit ventricular cells.

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Membrane potential fluctuations and transient inward currents induced by reactive oxygen intermediates in isolated rabbit ventricular cells.

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider