Left anterior descending coronary artery ligation in mice induced progressive cardiac remodelling, with scar formation completed by day 21 and progressive LV dilation up to 6 months.
This preclinical study maps the precise timeline of cellular infiltration, scar formation, and progressive left ventricular remodeling up to 6 months following induced myocardial infarction in a mouse model.
We established a mouse model of cardiac dysfunction due to myocardial infarction (MI). For this we ligated the left anterior descending coronary artery in male C57BL/6J mice and assessed healing and left ventricular (LV) remodelling at 1, 2 and 4 days and 1, 2 and 4 weeks after MI. Echocardiography was performed at 1 and 2 weeks and 1, 2, 4 and 6 months after MI. We found that neutrophil infiltration of the infarct border was noticeable at 1-2 days. Marked macrophage infiltration occurred at day 4, while lymphocyte infiltration was apparent at 7-14 days. Massive proliferation of fibroblasts and collagen accumulation began by day 7-14, and scar formation was completed by day 21. LV diastolic dimension increased markedly at 2 weeks and remained at the same level thereafter. LV shortening fraction decreased significantly at 2 weeks and then slowly decreased. In non-infarcted areas of the LV, myocyte cross-sectional area and interstitial collagen fraction increased progressively, reaching a maximum at 4 months. This study provides important qualitative and quantitative information about the natural history of cardiac remodelling after MI in mice.
Yang et al. (2002) studied Myocardial infarction. Left anterior descending coronary artery ligation was evaluated on Healing and left ventricular remodelling. Left anterior descending coronary artery ligation in mice induced progressive cardiac remodelling, with scar formation completed by day 21 and progressive LV dilation up to 6 months.