What question did this study set out to answer?

To examine the expression of troponin I isoforms throughout human cardiac development and in end-stage heart failure.

May 1, 1993Circulation ResearchOpen Access

Troponin I gene expression during human cardiac development and in end-stage heart failure.

Q: What is the clinical evidence from this study?

Study design: Observational. Population: Cardiac development and end-stage heart failure. Intervention: Cardiac development and heart failure vs. Fetal vs postnatal; normal vs failing heart. Primary outcome: Troponin I isoform expression (cardiac vs slow skeletal muscle troponin I).

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Key result

Slow skeletal muscle troponin I is replaced by cardiac troponin I by 9 months postnatally, and the skeletal isoform is not re-expressed in adult end-stage heart failure.

Authors

SSSarah K. SasseNational Jewish Health NBNigel J. BrandCentre National de la Recherche Scientifique PKPhillip KyprianouUniversity of London

Discussion

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Implication

No basis to invoke skeletal troponin I re-expression in adult HF; extends developmental isoform data but leaves clinical relevance open.

Key Points

To examine the expression of troponin I isoforms throughout human cardiac development and in end-stage heart failure.
Analyzed heart samples from 9 weeks gestation to 9 months postnatal life using Western and Northern blots.
Studied 17 hearts with end-stage heart failure from various causes.
Measured presence and abundance of troponin I isoforms and their mRNAs.
Slow skeletal muscle troponin I predominates during fetal life.
Cardiac troponin I becomes the only detectable isoform by 9 months postnatally.
Troponin I mRNA levels correlate with protein isoform patterns; slow skeletal muscle troponin I mRNA is absent in heart failure.

Study Design

Type

Observational

PICO

Population

Human heart samples from 9 weeks of gestation to 9 months of postnatal life, and 17 adult hearts with end-stage heart failure, analyzed for troponin I isoform expression.

Exposure / Comparator

Cardiac development and heart failure vs Fetal vs postnatal; normal vs failing heart

Primary Outcome

Troponin I isoform expression (cardiac vs slow skeletal muscle troponin I)

Cite This Study

Sasse et al. (1993) conducted an observational in Cardiac development and end-stage heart failure. Cardiac development and heart failure vs. Fetal vs postnatal; normal vs failing heart was evaluated on Troponin I isoform expression (cardiac vs slow skeletal muscle troponin I). Slow skeletal muscle troponin I is replaced by cardiac troponin I by 9 months postnatally, and the skeletal isoform is not re-expressed in adult end-stage heart failure.

synapsesocial.com/papers/6a22c90ca1bd7ea8dd11db69 https://doi.org/10.1161/01.res.72.5.932

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Troponin I gene expression during human cardiac development and in end-stage heart failure.

Discussion

Member takes

Implication

Key Points

Study Design

PICO

Cite This Study

Troponin I gene expression during human cardiac development and in end-stage heart failure.

Discussion

Member takes

Implication

Key Points

Study Design

PICO

Cite This Study

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