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Functional expression of cloned cDNA encoding sodium channel III | Synapse

February 8, 1988FEBS Letters163 citationsOpen Access

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Functional expression of cloned cDNA encoding sodium channel III

HSHarukazu SuzukiRIKEN Center for Integrative Medical Sciences SBSynnöve BeckhMax Planck Society HKHideo KuboForge Nano (United States)

Key Points

This research aims to investigate the functional expression of cloned cDNA for sodium channel III.
In vitro transcription of cloned cDNA to synthesize mRNA.
Formation of functional sodium channels in Xenopus oocytes using the synthesized mRNA.
Blot hybridization analysis to study tissue distribution of sodium channel mRNAs.
Sodium channel III mRNA directs the formation of a functional sodium channel.
Tissue distribution of mRNAs for sodium channels I, II, and III was successfully mapped.

Abstract

mRNA synthesized by transcription in vitro of the cloned cDNA encoding rat brain sodium channel III directs the formation of a functional sodium channel in Xenopus oocytes. The tissue distribution of the mRNAs encoding sodium channels I, II and III has been studied by blot hybridization analysis with specific probes.

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Cite This Study

Suzuki et al. (Mon,) studied this question.

synapsesocial.com/papers/6a231e1e4a04428ad5657576 https://doi.org/https://doi.org/10.1016/0014-5793(88)80615-x