What question did this study set out to answer?

This research investigates how endothelin enhances contractility in heart cells, focusing on its effect on intracellular pH.

January 1, 1991Circulation ResearchOpen Access

Endothelin and increased contractility in adult rat ventricular myocytes. Role of intracellular alkalosis induced by activation of the protein kinase C-dependent Na(+)-H+ exchanger.

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Population

Isolated adult rat ventricular cells loaded with the H(+)-selective fluorescent probe BCECF

Comparison

Endothelin superfusion with or without inhibitors vs Baseline

Design

Preclinical

Authors

BKBernhard K. KrämerUniversity of Mannheim TSThomas W. SmithBoston University RKR A KellyAstraZeneca (United Kingdom)

Discussion

Member takes

Implication

Endothelin boosts rat myocyte contractility partly via PKC-NHE alkalinization; leaves open human relevance and therapeutic targeting.

Key Points

This research investigates how endothelin enhances contractility in heart cells, focusing on its effect on intracellular pH.
Isolated adult rat ventricular myocytes were used, loaded with the H(+)-sensitive probe BCECF.
Endothelin was applied at varying concentrations (100 pM and 1 nM), and changes in contractility and intracellular pH were measured.
Effects of amiloride and protein kinase C inhibitors on endothelin's actions were assessed.
Endothelin at 100 pM increased contractile amplitude to 190 +/- 26% of baseline and pHi by 0.08 +/- 0.02 (n=8).
1 nM endothelin raised pHi by 0.13 +/- 0.03 with minimal additional contractile increase.
Amiloride reduced the inotropic response by 45%, while protein kinase C inhibitors similarly diminished endothelin's effects.

Structured PICO

Population

Isolated adult rat ventricular cells loaded with the H(+)-selective fluorescent probe BCECF

Intervention

Endothelin superfusion (100 pM and 1 nM) with or without inhibitors (amiloride, H-7, sphingosine, pertussis toxin)

Comparator

Baseline

Outcome

Contractile amplitude and intracellular pH (pHi)surrogate

The positive inotropic effect of endothelin in rat ventricular myocytes is partially mediated by intracellular alkalinization via PKC-dependent activation of the Na(+)-H+ exchanger.

Cite This Study

Krämer et al. (1991) studied this question.

synapsesocial.com/papers/6a23bd859e1c90a91c092c04 https://doi.org/10.1161/01.res.68.1.269

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Endothelin and increased contractility in adult rat ventricular myocytes. Role of intracellular alkalosis induced by activation of the protein kinase C-dependent Na(+)-H+ exchanger.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Endothelin and increased contractility in adult rat ventricular myocytes. Role of intracellular alkalosis induced by activation of the protein kinase C-dependent Na(+)-H+ exchanger.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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