Key result
A small 5' open reading frame in the C/EBP alpha mRNA dictates translation start site multiplicity by causing ribosomes to bypass the first AUG codon via leaky scanning.
Population
Chicken, rat, and Xenopus C/EBP alpha mRNA models, COS-1 cells, LMH cells, and chicken tissues
Comparison
Mutation of the small 5'ORF and Kozak sequences… vs Wild-type cC/EBP alpha expression plasmids
Design
Preclinical
Authors
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Reveals evolutionary conservation of C/EBP start-site control; leaves open isoform-specific roles in mammalian physiology and disease.
The study demonstrates that a conserved small 5' open reading frame dictates translation start site multiplicity in C/EBP alpha mRNA via a leaky scanning mechanism.
Calkhoven et al. (1994) studied this question. C/EBP alpha mRNA 5' ORF mutation vs. Wild-type C/EBP alpha mRNA was evaluated on Translation start site multiplicity (isoform ratio). A small 5' open reading frame in the C/EBP alpha mRNA dictates translation start site multiplicity by causing ribosomes to bypass the first AUG codon via leaky scanning.
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