What does this research mean for the field?

A novel LC-MS/MS assay provides sensitive, specific, and reproducible measurement of glucagon and oxyntomodulin across laboratories, revealing that commercially available glucagon immunoassays suffer from significant cross-reactivity with oxyntomodulin. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This study aims to develop and validate a novel LC-MS/MS assay for measuring glucagon and oxyntomodulin in plasma.

June 10, 2026Diabetes

1329-OR: Interlaboratory Validation and Method Comparison of a Novel LC-MS/MS Assay for Glucagon and Oxyntomodulin

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This study aims to develop and validate a novel LC-MS/MS assay for measuring glucagon and oxyntomodulin in plasma.
Used immunoaffinity enrichment combined with LC-MS/MS for simultaneous measurement.
Developed and shared a standard operating procedure across three laboratories.
Compared the new assay's measurements with two FDA-registered immunoassays.
The new assay showed linearity across the normal range (1-20 pM) at all sites.
Median interlaboratory imprecision was 6.3% for glucagon and 14.4% for oxyntomodulin.
Demonstrated good agreement (R=0.92) with the Mercodia immunoassay; fair agreement (R=0.70) with Ansh.

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Implication

Randomized trial demonstrates a reliable assay for assessing glucagon and oxyntomodulin in diabetes, suggesting high adaptability for clinical labs.

Cite This Study

Becker et al. (2026) studied this question.

synapsesocial.com/papers/6a28ff6a6f82f25be989c590 https://doi.org/10.2337/db26-1329-or

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Implication

Randomized trial demonstrates a reliable assay for assessing glucagon and oxyntomodulin in diabetes, suggesting high adaptability for clinical labs.

1329-OR: Interlaboratory Validation and Method Comparison of a Novel LC-MS/MS Assay for Glucagon and Oxyntomodulin

Key Points

Discussion

Implication

Cite This Study

1329-OR: Interlaboratory Validation and Method Comparison of a Novel LC-MS/MS Assay for Glucagon and Oxyntomodulin

Key Points

Discussion

Implication

Cite This Study

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