What is the clinical evidence from this study?

Population: Diabetes. Intervention: Alloxan- and streptozotocin-induced diabetes vs. Control rats. Primary outcome: Rate of ATP-dependent tris-oxalate facilitated Ca2+ transport.

What question did this study set out to answer?

This study aims to understand how diabetes affects calcium transport in the cardiac sarcoplasmic reticulum of rats and the role of long chain acylcarnitines.

May 1, 1983Canadian Journal of Physiology and Pharmacology

The effect of alloxan- and streptozotocin-induced diabetes on calcium transport in rat cardiac sarcoplasmic reticulum. The possible involvement of long chain acylcarnitines

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Key result

In 120-day diabetic rats, cardiac sarcoplasmic reticulum showed a significant decrease in ATP-dependent Ca2+ transport (62-73% of control), possibly due to buildup of long chain acylcarnitines.

Key Points

This study aims to understand how diabetes affects calcium transport in the cardiac sarcoplasmic reticulum of rats and the role of long chain acylcarnitines.
Diabetes was induced in female Wistar rats using alloxan or streptozotocin.
Calcium transport was assessed in isolated working hearts and sarcoplasmic reticulum from these rats at different time points (7 days and 120 days post-induction).
ATP-dependent calcium transport and acylcarnitine levels were measured in rat cardiac preparations.
Cardiac sarcoplasmic reticulum from 120-day diabetic rats showed a decrease in Ca2+ transport rate (62-73% of control) and Ca2+ ATPase activity.
Higher levels of long chain acylcarnitines were found in 120-day diabetic rats compared to controls, with palmitylcarnitine acting as a potent inhibitor.
No significant change in calcium transport was found in 7-day diabetic rats, suggesting chronic alterations emerge over time.

Structured PICO

Population

Female Wistar rats evaluated 7 or 120 days after induction of diabetes by alloxan or streptozotocin.

Exposure

Induction of diabetes by a single I.V. injection of either alloxan (65 mg/kg) or streptozotocin (60 mg/kg)

Comparator

Control rats

Outcome

Rate of ATP-dependent tris-oxalate facilitated Ca2+ transport in cardiac sarcoplasmic reticulumsurrogate

Chronic (120-day) but not acute (7-day) experimental diabetes in rats impairs cardiac sarcoplasmic reticulum calcium transport, potentially due to the accumulation of long chain acylcarnitines.

Discussion

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Implication

Hypothesis-generating for acylcarnitine-mediated SR dysfunction in chronic diabetes; leaves open relevance to human cardiomyopathy.

Cite This Study

Lopaschuk et al. (1983) studied Diabetes. Alloxan- and streptozotocin-induced diabetes vs. Control rats was evaluated on Rate of ATP-dependent tris-oxalate facilitated Ca2+ transport. In 120-day diabetic rats, cardiac sarcoplasmic reticulum showed a significant decrease in ATP-dependent Ca2+ transport (62-73% of control), possibly due to buildup of long chain acylcarnitines.

synapsesocial.com/papers/6a2990ebdd2fa14b4da357cc https://doi.org/10.1139/y83-068

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The effect of alloxan- and streptozotocin-induced diabetes on calcium transport in rat cardiac sarcoplasmic reticulum. The possible involvement of long chain acylcarnitines

Key Points

Structured PICO

Discussion

Implication

Cite This Study

The effect of alloxan- and streptozotocin-induced diabetes on calcium transport in rat cardiac sarcoplasmic reticulum. The possible involvement of long chain acylcarnitines

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider