Endothelin antiserum decreases volume-stimulated and basal plasma concentration of atrial natriuretic peptide.
Key result
Intravenous injection of endothelin-1 antiserum in anesthetized rats reduced basal plasma ANP concentration and attenuated volume-stimulated ANP release compared to control serum (P<0.01).
Why the study?
Does intravenous injection of antiserum against ET-1 decrease basal and volume-stimulated plasma concentrations of ANP in anesthetized rats?
Population
Pentobarbital-anesthetized Wistar rats
Comparison
Intravenous injection of antiserum against ET-1 vs Control rat serum
Design
Preclinical
Follow-up
60 minutes
Authors
Discussion
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Implication
Findings in rats suggest ET-1 modulates ANP release; hypothesis-generating and should not change practice.
Key Points
- This research aims to understand how endogenous endothelin-1 influences the release of atrial natriuretic peptide.
- Used passive immunization with endothelin-1 antiserum in anesthetized Wistar rats
- Measured plasma concentrations of atrial natriuretic peptide using radioimmunoassay
- Assessed the impact of intravenous saline infusion on plasma ANP levels among different treatment groups.
- Plasma ANP concentration decreased by 37% after 10 minutes and 30% after 60 minutes following antiserum treatment
- Control serum showed no significant impact on plasma ANP levels
- Saline infusion caused a sixfold increase in plasma ANP among control rats, but only a twofold increase in ET-1 antiserum treated rats (P < .01).
Structured PICO
Does intravenous injection of antiserum against ET-1 decrease basal and volume-stimulated plasma concentrations of ANP in anesthetized rats?
P
Population
Pentobarbital-anesthetized Wistar rats used to test the role of endogenous ET-1 in the regulation of ANP release.
I
Intervention
Intravenous injection of antiserum against ET-1
C
Comparator
Control rat serum
O
Outcome
Plasma ANP concentration (basal and volume-stimulated)surrogate
Main Result
p-value: p=<.01
Endogenous endothelin-1 acts as a physiological modulator of both basal and volume-stimulated atrial natriuretic peptide release in vivo.
Cite This Study
Fyhrquist et al. (1993) studied Anesthetized rats. Endothelin-1 antiserum vs. Control serum was evaluated on Plasma ANP concentration (basal and volume-stimulated) (p=<.01). Intravenous injection of endothelin-1 antiserum in anesthetized rats reduced basal plasma ANP concentration and attenuated volume-stimulated ANP release compared to control serum (P<0.01).