Increasing carbohydrate oxidation improves contractile reserves and prevents hypertrophy in porcine right heart failure

Figures

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  Figure 1. Overview of the cytosolic and mitochondrial carbohydrate metabolic pathways involved in the progression of heart failure. The illustrated pathways are the major carbohydrate sources of the energy that is ultimately needed for myocardial contraction, and they thus represent potential targets for future metabolic therapy. Fatty-acid metabolism is omitted. GLUT = glucose transporters, LDH = lactate dehydrogenase, PDH = pyruvate dehydrogenase.

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  Figure 2. Dichloroacetate (DCA) attenuates hypertrophy and contractile impairment in pigs with right ventricular (RV) volume overload (RVO). (a): Atrial natriuretic peptide (ANP) in plasma. (b + c): CINE MRI shows dilation of the right ventricle after 19 weeks of overload, quantified as the end-diastolic volume (EDV). The white scale bars are 1 cm. (d) RV mass from MRI. (e) RV wall thickness ex-vivo after fixation. (f + g): MRI determined change in systolic function from rest to stress induced with dobutamine, pp indicates percentage points. Error bars indicate 95% confidence intervals. NS: P > 0.1, *P < 0.05, **P < 0.01, ***P < 0.001, (g) was tested with Kruskal-Wallis, (a + c-f) with ANOVA with Benjamini-Hochberg correction.

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  Figure 3. Dichloroacetate (DCA) reverts the deterioration of myocardial pyruvate metabolism after chronic right ventricular overload (RVO) in pigs. (a) We assessed metabolism with hyperpolarized [1-13C]pyruvate magnetic resonance spectroscopy under rest and dobutamine-induced stress. This technique allows assessment of flux through the pyruvate dehydrogenase (PDH), the lactate dehydrogenase (LDH) and the alanine transaminase (ALT). (b) The lactate and bicarbonate production of the heart after injection of hyperpolarized pyruvate. (c) The metabolism quantified using a model-free approach. The asterisks indicate significance vs. controls. Only significant comparisons are shown for display purposes. (d) Myocardial pH modelled from the spectroscopy data. e: In-vitro enzyme activity. Error bars and ribbons indicate 95% confidence intervals. NS: P > 0.1, *P < 0.05, **P < 0.01. ***P < 0.001, tested with ANOVA with Benjamini-Hochberg correction.

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  Figure 4. Dichloroacetate (DCA) increases mitochondrial respiratory function after right ventricular volume overload (RVO) in pigs. (a) The respiration rates of isolated cardiac mitochondria in a closed-chamber system with pyruvate and malate as substrates after 19 weeks of overload. (b) The maximal in-vitro mitochondrial state 3 oxygen consumption rates (OCR) correlates with bicarbonate production as determined with hyperpolarized [1-13C]pyruvate magnetic resonance spectroscopy (MRS) under dobutamine-induced stress. (c) Citrate synthase activity in the isolated mitochondria. (d) Thiobarbituric acid reactive substances (TBARS), an end-product of oxidative stress, in the overloaded right ventricle. Error bars indicate 95% confidence intervals. NS: P > 0.1, * P < 0.05, ** P < 0.01. *** P < 0.001, ANOVA with Benjamini-Hochberg correction. Correlation was assessed with Pearson’s method.