Analysis of Epidemiological and Molecular Characteristics of Bocavirus in Guangzhou

Figures

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  Figure 1. Seasonal distribution (A) and age distribution (B) of bocavirus in Guangzhou. Mark * indicates a comparison between age groups with p < 0.005.

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  Figure 2. Detection of co-infection viruses with bocavirus (A) and age distribution of cases co-infected with bocavirus (B).

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  Figure 3. Phylogenetic analysis of human bocavirus whole genome. Mark ◆ denotes sequences obtained in this study.

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  Figure 4. Phylogenetic analysis based on full length (A), NS1 (B), NP1 (C), and VP1/VP2 (D) gene. Mark ◆ denotes sequences obtained in our study. Mark ● denotes sequences obtained in this study for which it is speculated that gene recombination has occurred.

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  Figure 5. Simplot recombination detection results (A,B) and phylogenetic tree based on breakpoint partition sequences (C–G). Mark ▲ and ▼ denote suspected recombinant sequences GZ-2024-20891 and GZ-2025-19422, respectively. (C–E) The phylogenetic trees of sequence GZ-2024-20891 segmented into three parts: the segment before the first breakpoint, the segment between the two breakpoints, and the segment after the second breakpoint, respectively. (F,G) The phylogenetic trees of sequence GZ-2025-19422 segmented into two parts: the segment before the breakpoint and the segment after the breakpoint, respectively.

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  Figure 6. Three-dimensional visualization of predicted B cell conformational epitopes on the VP2 protein. A represents the predicted model of the VP2 protein of the recombinant strain GZ-2024-20891; B and C represent the models of the VP2 protein of the parental strain LWK and Bonn-1, respectively; and D and E represent the models of the VP2 protein of the circulating strain GZ-2024-15663 and YN-1044. α denotes the front view, β denotes the left-side view, and γ denotes the top view.