Protective effect of nebivolol on doxorubicin-induced cardiotoxicity in rats

Figure 1. Photomicrograph of the left ventricle. A, B – control group showing the myocytes appeared cylindrical (arrow) with central oval nuclei (arrow heads) and blood capillaries (red arrow). C–F – subgroup I showing marked distortion (wavy arrows) and wide separation (stars) of cardiac myocytes. Some myocytes showed peripheral (yellow arrow) and pyknotic nuclei (red arrow). Inflammatory cell infiltrates (thick arrow), cytoplasmic vacuolation (V) of cardiac myocytes and massive congestion of the blood vessels (C) were evident. Lymph vessels were markedly dilated with acidophilic lymph (blue arrow). G, H – subgroup II showing regeneration of cardiac muscles fibers with few congested capillaries (C), (H + E, 400×)

Figure 2. Photomicrograph of the left ventricle. A – Control group showing scanty collagen fibers between the cardiac myocytes. B – Subgroup I showing increased collagen deposition between the cardiac myocytes. C – subgroup II showing relative reduction in collagen deposition between cardiac myocytes. Arrows: collagen fibers (Masson’s trichrome, 400×)

Figure 3. Photomicrograph of the left ventricle. A – Control group showing mild localized immunoreactivity in the sarcoplasm of cardiac myocytes. B – Subgroup I showing dense immunoreactivity in the sarcoplasm of cardiac myocytes. C – Subgroup II showing mild immunoreactivity in the sarcoplasm of cardiac myocytes. Arrows: immunoreactivity in sarcoplasm of cardiac myocytes (caspase-3, 400×)

Figure 4. Photomicrograph of the left ventricle. A – Control group showing mild immunoreactivity in the sarcoplasm of cardiac myocytes (arrow head) as well as in the blood vessel endothelium (arrow). B – Subgroup I showing increased immunoreactivity in the sarcoplasm of cardiac myocytes (arrows) as well as in the blood vessel endothelium (wavy arrow). C – Subgroup II showing reduced immunoreactivity in the sarcoplasm of cardiac myocytes (arrows) as well as in the blood vessel endothelium (wavy arrow) (eNOS, 400×)

Figure 5. Photomicrograph of the left ventricle. A – Control group showing mild localized immunoreactivity in the sarcoplasm of cardiac myocytes as well as in the blood vessel endothelium. B – Subgroup I showing dense immunoreactivity in the sarcoplasm of cardiac myocytes as well as in the blood vessel endothelium. C – Subgroup II showing mild immunoreactivity in the sarcoplasm of cardiac myocytes as well as in the blood vessel endothelium. Arrows: immunoreactivity in sarcoplasm of cardiac myocytes, arrow head: immunoreactivity in the blood vessel endothelium (iNOS, 400×)

Figure 6. Photomicrograph of the left ventricle. A – Control group showing mild localized immunoreactivity in the sarcoplasm of cardiac myocytes. B – Subgroup I showing dense diffuse immunoreactivity in the sarcoplasm of cardiac myocytes. C – Subgroup II showing reduced immunoreactivity in the sarcoplasm of cardiac myocytes. Arrows: immunoreactivity in sarcoplasm of cardiac myocytes (TNF-α, 400×)