Key result
In rabbit ventricular muscle, equilibration with 100 nM ryanodine virtually eliminated both Na-free caffeine contractures and rapid cooling contractures, indicating an inability of the SR to retain Ca.
Ryanodine prevents the sarcoplasmic reticulum from retaining calcium during Na-free perfusion in rabbit ventricular muscle, likely due to sarcolemmal calcium pump extrusion.
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Rabbit SR Ca retention during Na-free perfusion shown; leaves open translation to human Ca handling or arrhythmias.
Donald M. Bers (1987) studied this question. Ryanodine was evaluated on Sarcoplasmic reticulum calcium content assessed by caffeine and rapid cooling contractures. In rabbit ventricular muscle, equilibration with 100 nM ryanodine virtually eliminated both Na-free caffeine contractures and rapid cooling contractures, indicating an inability of the SR to retain Ca.
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