Key result
Ryanodine irreversibly decreased contraction amplitude by 10-70%, while caffeine caused a transient 5-12% increase followed by a sustained 9-76% decrease in contraction amplitude.
Population
Chick embryo ventricular cell monolayer cultures
Comparison
Ryanodine (10-10 M) and Caffeine vs Control
Design
Preclinical
Authors
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Effects in chick embryo cultures should not inform clinical use; leaves open ryanodine's Ca-handling role pending mammalian validation.
In cultured chick embryo ventricular cells, caffeine and ryanodine alter contractility and calcium fluxes, highlighting the important role of the sarcoplasmic reticulum in excitation-contraction coupling.
Rasmussen et al. (1987) studied this question. Ryanodine and caffeine vs. Control was evaluated on Changes in mechanical and electrical activity, 45Ca fluxes, and cell Ca contents. Ryanodine irreversibly decreased contraction amplitude by 10-70%, while caffeine caused a transient 5-12% increase followed by a sustained 9-76% decrease in contraction amplitude.
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