Key result
Moderate to severe COPD was associated with lower resting vastus lateralis ATP concentrations compared to healthy controls, alongside reduced GLUT4 and MCT4.
Why the study?
Do patients with moderate to severe COPD have altered energy metabolic and transporter characteristics in resting vastus lateralis muscle compared to healthy controls?
Case-Control (n=21)
Do patients with moderate to severe COPD have altered energy metabolic and transporter characteristics in resting vastus lateralis muscle compared to healthy controls?
Moderate to severe COPD is associated with reduced phosphorylation potential, elevated glycolytic flux, and potential defects in glucose and lactate transport in resting skeletal muscle.
Answers are grounded in this paper and cite their sources.
Hypothesis-generating for muscle metabolic impairment in COPD; larger prospective studies needed before clinical relevance.
To investigate energy metabolic and transporter characteristics in resting muscle of patients with moderate to severe chronic obstructive pulmonary disease [COPD; forced expiratory volume in 1 s (FEV(1)) = 42 +/- 6.0% (mean +/- SE)], tissue was extracted from resting vastus lateralis (VL) of 9 COPD patients and compared with that of 12 healthy control subjects (FEV(1) = 114 +/- 3.4%). Compared with controls, lower (P < 0.05) concentrations (mmol/kg dry wt) of ATP (19.6 +/- 0.65 vs. 17.8 +/- 0.69) and phosphocreatine (81.3 +/- 2.3 vs. 69.1 +/- 4.2) were observed in COPD, which occurred in the absence of differences in the total adenine nucleotide and total creatine pools. Higher concentrations were observed in COPD for several glycolytic metabolites (glucose-1-phosphate, glucose-6-phosphate, fructose-6-phosphate, pyruvate) but not lactate. Glycogen storage was not affected by the disease (289 +/- 20 vs. 269 +/- 20 mmol glucosyl units/kg dry wt). Although no difference between groups was observed for the glucose transporter GLUT1, GLUT4 was reduced by 28% in COPD. For the monocarboxylate transporters, MCT4 was 35% lower in COPD, with no differences observed for MCT1. These results indicate that in resting VL, moderate to severe COPD results in a reduction in phosphorylation potential, an apparent elevation of glycolytic flux rate, and a potential defect in glucose and lactate transport as a result of reduced levels of the principal isoforms.
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Green et al. (2008) conducted a case-control in chronic obstructive pulmonary disease (n=21). Moderate to severe COPD vs. Healthy control subjects was evaluated on ATP concentration (mmol/kg dry wt) (p=< 0.05). Moderate to severe COPD was associated with lower resting vastus lateralis ATP concentrations compared to healthy controls, alongside reduced GLUT4 and MCT4.
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