What is the clinical evidence from this study?

Study design: Other. Population: Diabetes mellitus. Intervention: Streptozotocin-induced diabetes mellitus vs. Controls. Primary outcome: Ca2+ pump activity (Ca2+-stimulated adenosine triphosphatase and Ca2+-uptake ability).

What question did this study set out to answer?

This research aims to investigate the activity of the cardiac sarcolemmal Ca2+ pump in the context of diabetes mellitus.

March 1, 1987AJP Heart and Circulatory Physiology

Alterations in cardiac sarcolemmal Ca2+ pump activity during diabetes mellitus

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Key result

Streptozotocin-induced diabetes mellitus in rats significantly depressed cardiac sarcolemmal Ca2+ pump activity compared to controls.

Population

Male Wistar rats, 8 weeks after streptozotocin injection (55 mg/kg iv) to induce diabetes mellitus

Comparison

Streptozotocin-induced diabetes mellitus vs Non-diabetic control rats

Design

Preclinical

Follow-up

8 weeks

Authors

CHClayton E. HeyligerRoss University School of Veterinary Medicine APAllanR. PrakashUniversity of British Columbia JMJohn H. McNeillHeart Failure / Cardiomyopathy

Discussion

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Implication

Should not change clinical practice; leaves open whether sarcolemmal Ca2+ pump dysfunction contributes to human diabetic cardiomyopathy.

Key Points

This research aims to investigate the activity of the cardiac sarcolemmal Ca2+ pump in the context of diabetes mellitus.
Isolation of purified sarcolemmal membranes from male Wistar diabetic rats 8 weeks post-streptozotocin injection (55 mg/kg iv)
Assessment of Ca2+ pump activity through Ca2+-stimulated adenosine triphosphatase and Ca2+-uptake capabilities
Evaluation of the impact of calmodulin on Ca2+ pump activity
Ca2+ pump activity was significantly depressed in the diabetic myocardium compared to controls
Findings were not influenced by contamination from the sarcoplasmic reticulum or mitochondria
Indicates that the defective sarcolemmal Ca2+ pump contributes to altered Ca2+ transport in diabetes mellitus.

Structured PICO

Population

Male Wistar rats with streptozotocin-induced diabetes mellitus evaluated 8 weeks post-injection.

Exposure

Streptozotocin-induced diabetes mellitus

Comparator

Non-diabetic control rats

Outcome

Cardiac sarcolemmal Ca2+ pump activity (Ca2+-stimulated adenosine triphosphatase and Ca2+-uptake ability in the absence and presence of calmodulin)surrogate

The sarcolemmal Ca2+ pump is defective in the diabetic myocardium, which may contribute to altered Ca2+ transport and the development of diabetic cardiomyopathy.

Cite This Study

Heyliger et al. (1987) studied Diabetes mellitus. Streptozotocin-induced diabetes mellitus vs. Controls was evaluated on Ca2+ pump activity (Ca2+-stimulated adenosine triphosphatase and Ca2+-uptake ability). Streptozotocin-induced diabetes mellitus in rats significantly depressed cardiac sarcolemmal Ca2+ pump activity compared to controls.

synapsesocial.com/papers/6a64b13a7661d0b8adcfbc74 https://doi.org/10.1152/ajpheart.1987.252.3.h540

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Alterations in cardiac sarcolemmal Ca2+ pump activity during diabetes mellitus

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Alterations in cardiac sarcolemmal Ca2+ pump activity during diabetes mellitus

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider