Cardiac 18F-FDG Positron Emission Tomography: An Accurate Tool to Monitor In vivo Metabolic and Functional Alterations in Murine Myocardial Infarction

Figures

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  Figure 1. Histological and PET imaging of murine myocardial infarction. (A) Histology section of the heart 30 days after LAD ligation. HE-Staining (left) and Masson's trichrome staining (right). Stars and arrows indicate the magnified infarct scare area in the left ventricular (LV) wall (blue). Right ventricle (RV). Bar equals 100 and 50 μm, respectively. (B) Representative bulls-eye image of the left ventricle of healthy (upper image) and infarcted left ventricle (lower image) showing the defect of the infarct. Color Scale from QPS: Cool and in percentage range. (C) Panel shows PET images of three representative infarct hearts (left, middle, and right panel) each in end-diastole (ED) and end-systole (ES) in different axes at various levels of the left ventricle. ED volume (green line) and ES volume (red line) depicting the motion deficit in the infarct area (KA, coronary axis; VLA, vertical long axis; HLA, horizontal long axis). EF% from left to right: 40%, 34%, 42% (QGS); Defect% (QPS) from left to right: 48%, 44%, 40%. Color Scale from QGS: Cool as used in (B). For the segmental defect and motion deficit of the three representative mice, please see Supplementary Figures 2A,B,…

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  Figure 2. Scatter plots of the correlation between histological and PET infarct areas. (A) Cumulative correlations between histological and PET infarct area (in %). Corresponding Bland-Altman-plot on the right side. Dotted lines display the Limits of agreement (LoA) and correlation line. Dashed lines indicate bias. (B) Correlations between histological and PET infarct area (in %) at day 3 post-MI. Corresponding Bland-Altman-plot on the right side. (C) Correlations between histological and PET infarct area (in %) at day 30 post-MI. Corresponding Bland-Altman-plot on the right side.

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  Figure 3. Alterations in LVMV and cardiac %ID/g in myocardial infarction in 10 weeks old mice (A) displays the LVMV and (B) for cardiac %ID/g at baseline, 3, 14- and 30-days post-MI. All groups: n = 4-16. All data represent mean ± SD. * p = 0.05, ** p < 0.01, *** p < 0.001.

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  Figure 4. Evaluation of cardiac function after myocardial injury in comparison to age-matched littermates (A) illustrates three-dimensional reconstruction of the left ventricular EDV in the ROA view. Upper picture: EDV at baseline and lower picture at 30 days post-LAD ligation. (B) Comparison of cardiac PET parameters at baseline, 3, and 30 days post-MI. Data of healthy age-matched littermates are illustrated in green, and infarct mice are in red. All groups: n = 6-16. Data represent mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.