Key result
Mutant influenza A nucleoproteins expressed at nonpermissive temperatures were defective for RNA binding, suggesting this activity is required for replicative transcription.
Population
Influenza A virus strains (A/WSN/33 ts56, A/FPV/Rostock/34/Giessen tsG81, A/PR/8/34) and transfected cells
Comparison
Introduction of temperature-sensitive point… vs Wild-type NP or expression at permissive…
Design
Preclinical
Authors
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NP RNA-binding defects in ts mutants impair cRNA synthesis; leaves open how NP regulates the mRNA-cRNA switch in vivo.
The study demonstrates that the RNA-binding activity of the influenza virus nucleoprotein is essential for replicative transcription.
Medcalf et al. (1999) studied Influenza A virus. Temperature-sensitive mutations in the nucleoprotein (NP) coding region vs. Permissive temperature (30 degrees C) was evaluated on RNA binding activity and replicative transcription. Mutant influenza A nucleoproteins expressed at nonpermissive temperatures were defective for RNA binding, suggesting this activity is required for replicative transcription.
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