Temperature-sensitive mutants of influenza WSN virus defective in virus-specific RNA synthesis
Key result
Influenza WSN virus mutants in complementation groups I and III ceased cRNA synthesis at 39.5 C, indicating a defect in the transcriptase complex, unlike groups II and V.
Population
Influenza WSN virus temperature-sensitive mutants and infected cells
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Reveals essential roles for groups I and III in cRNA synthesis; leaves open their validation in broader replication models.
Structured PICO
P
Population
Influenza WSN virus temperature-sensitive (ts) mutants (complementation groups I, II, III, and V) and infected cells
I
Intervention
Incubation at nonpermissive temperature (39.5 C) and temperature shift-up from permissive (33 C) to nonpermissive (39.5 C)
O
Outcome
Viral complementary RNA (cRNA) synthesis
The study identifies that two influenza viral gene functions are required for transcription and likely two additional functions for RNA replication based on temperature-sensitive mutants.
Cite This Study
Krug et al. (1975) studied Influenza WSN virus infection. Temperature-sensitive (ts) mutants of influenza WSN virus vs. Permissive temperature (33 C) was evaluated on Viral complementary RNA (cRNA) synthesis. Influenza WSN virus mutants in complementation groups I and III ceased cRNA synthesis at 39.5 C, indicating a defect in the transcriptase complex, unlike groups II and V.