Identification of the defective genes in three mutant groups of influenza virus
Key result
RNA polyacrylamide gel analysis identifies P2, nucleoprotein, and M proteins as defective in influenza mutants.
Why the study?
The defective genes in three mutant groups of temperature-sensitive influenza WSN virus mutants remained unidentified.
Population
Temperature-sensitive influenza WSN virus mutants groups II, V, and VII
Comparison
Wild-type recombinants versus ts mutants and different non-ts influenza viruses
Design
Preclinical study using RNA polyacrylamide gel analysis
Authors
Discussion
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Member takes
Implication
Reports defects in three remaining ts influenza groups; leaves open complete gene-function mapping for WSN virus.
Structured PICO
P
Population
Temperature-sensitive (ts) influenza WSN virus mutants (groups II, V, and VII) and wild-type recombinants
I
Intervention
RNA polyacrylamide gel analysis
O
Outcome
Identification of defective gene products in mutant groups II, V, and VIIsurrogate
Identified the defective genes (P2, nucleoprotein, M protein) in three temperature-sensitive mutant groups of influenza WSN virus, suggesting P2 and nucleoprotein are required for virion RNA synthesis.
Cite This Study
Ritchey et al. (1977) studied Influenza virus. RNA polyacrylamide gel analysis was evaluated on Identification of defective gene products. RNA polyacrylamide gel analysis identified the P2 protein, nucleoprotein, and M protein as the defective gene products in influenza virus mutant groups II, V, and VII, respectively.