In-Vivo and In-Vitro Conversion of Angiotensin I to Angiotensin II in Dog Blood
Key result
Injection of angiotensin I into the pulmonary artery resulted in 53% conversion to angiotensin II, indicating the pulmonary capillary bed is the major site of conversion in dogs.
Population
Intact anesthetized dogs and dog whole blood and plasma treated with various anticoagulants
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Supports blood and pulmonary Ang I-II conversion; leaves open dominant site and physiologic relevance in humans.
Structured PICO
P
Population
Intact anesthetized dogs (in vivo) and dog whole blood and plasma treated with various anticoagulants (in vitro)
I
Intervention
Injection of angiotensin I into the pulmonary artery (at 10,000 times physiologic level) or renal artery (1000 to 10,000 times physiologic level)
O
Outcome
Conversion of angiotensin I to angiotensin II (measured by fractionation of radioactively labeled peptides and radioimmunoassay)surrogate
This foundational preclinical study established that the pulmonary capillary bed, rather than plasma, is the primary physiological site for the conversion of angiotensin I to angiotensin II.
Cite This Study
Oparil et al. (1970) studied this question. Angiotensin I injection was evaluated on Conversion of angiotensin I to angiotensin II. Injection of angiotensin I into the pulmonary artery resulted in 53% conversion to angiotensin II, indicating the pulmonary capillary bed is the major site of conversion in dogs.