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October 15, 1993Proceedings of the National Academy of SciencesOpen Access

Cloning and expression of a Kv1.2 class delayed rectifier K+ channel from canine colonic smooth muscle.

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Key result

CSMK1, a Kv1.2 class delayed rectifier K+ channel cloned from canine colonic smooth muscle, showed a single-channel conductance of 14 pS and high sensitivity to 4-aminopyridine (IC50 74.7 µM).

Population

Canine colonic circular smooth muscle tissue and Xenopus laevis oocytes used for functional expression.

Design

Preclinical

Authors

PHPádraig HartBelfast Health and Social Care TrustKOKen OverturfAgricultural Research ServiceSRScott N. RussellUniversity of Nevada, Reno

Discussion

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Implication

Cloned GI-specific Kv channel offers research tool for motility; leaves open human validation and clinical relevance.

Structured PICO

P
Population
Basic science study investigating the cloning and expression of a Kv1.2 class delayed rectifier K+ channel (CSMK1) from canine colonic smooth muscle in Xenopus oocytes.
I
Intervention
Cloning and functional expression of CSMK1 cDNA (a Kv1.2 class delayed rectifier K+ channel).
O
Outcome
Electrophysiological properties, pharmacological sensitivity (e.g., to 4-aminopyridine), and tissue distribution of the CSMK1 channel.

The cloning and characterization of CSMK1 reveals a delayed rectifier K+ channel uniquely expressed in gastrointestinal smooth muscle that may regulate electrical slow wave activity.

Limitations

  • Difficult to dissect and characterize all of the currents expressed from mixed whole-cell current records in native cells

Cite This Study

Hart et al. (1993) studied this question. CSMK1 cloning and expression was evaluated on Electrophysiological and pharmacological properties of CSMK1. CSMK1, a Kv1.2 class delayed rectifier K+ channel cloned from canine colonic smooth muscle, showed a single-channel conductance of 14 pS and high sensitivity to 4-aminopyridine (IC50 74.7 µM).

synapsesocial.com/papers/6a6bd06426cff2e2f6f48341https://doi.org/10.1073/pnas.90.20.9659
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Cloning and expression of a rat cardiac delayed rectifier potassium channel.1991 · 72 citations
  2. 2Participation of fast-activating, voltage-dependent K currents in electrical slow waves of colonic circular muscle1992 · 57 citations
  3. 3Ionic currents in single smooth muscle cells of the canine renal artery.1992 · 80 citations
  4. 4Outward currents in longitudinal colonic muscle cells contribute to spiking electrical behavior1992 · 31 citations
  5. 5Effects of tetraethylammonium and 4‐aminopyridine on outward currents and excitability in canine tracheal smooth muscle cells1990 · 64 citations