What question did this study set out to answer?

This research aims to determine the role of apoptosis in myocyte loss during acute coxsackievirus myocarditis.

April 1, 1998Cardiovascular ResearchOpen Access

Expression of apoptosis-related proteins in experimental coxsackievirus myocarditis

Q: What is the clinical evidence from this study?

Study design: Other. Population: Acute viral myocarditis (n=40). Intervention: Coxsackievirus B3 (CVB3) inoculation vs. Saline vehicle-injected controls. Primary outcome: Myocardial apoptosis (TUNEL-positive cells).

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Key result

Inoculation with coxsackievirus B3 in mice resulted in marked inflammatory activity but exceedingly rare myocyte apoptosis in the myocardium.

Why the study?

Does coxsackievirus B3 infection induce myocyte apoptosis in experimental myocarditis?

Population

CD1 and C3H.HeJ (C3H) mice (5 mice/group)

Comparison

Inoculation with 5 x 10 pfu coxsackievirus B3 vs Saline vehicle-injected controls

Design

Preclinical

Follow-up

up to 3 weeks

Authors

JCJulia ColstonNorth Bristol NHS Trust

Discussion

Member takes

Implication

Apoptosis may contribute to myocyte loss in viral myocarditis; leaves open whether modulating it alters outcomes in patients.

Key Points

This research aims to determine the role of apoptosis in myocyte loss during acute coxsackievirus myocarditis.
Mice inoculated with coxsackievirus B3 (CVB3) were sacrificed at 1, 2, and 3 weeks post-inoculation (p.i.) for analysis.
Histopathology and TUNEL assays were used to quantify inflammation, necrosis, and apoptosis.
Immunoreactive staining was performed for apoptosis-related proteins in myocardium.
Significant histopathological changes were observed in both mouse strains at all time points.
Rare myocardial TUNEL-positive cells indicated minimal apoptosis despite inflammation observed.
Anti-apoptotic protein Bcl-2 showed increased immunoreactivity post-infection, correlating with histopathological scores.

Structured PICO

Does coxsackievirus B3 infection induce myocyte apoptosis in experimental myocarditis?

Population

CD1 and C3H.HeJ mice inoculated with coxsackievirus B3 to assess apoptosis in acute viral myocarditis over a 3-week period.

Intervention

Inoculation with 5 x 10(6) pfu coxsackievirus B3 (CVB3)

Comparator

Saline vehicle-injected controls

Outcome

Inflammation, necrosis, and apoptosis in myocardium quantified by histopathological status and TUNEL assayssurrogate

In experimental coxsackievirus B3 myocarditis, myocyte cell loss is primarily non-apoptotic, accompanied by an upregulation of anti-apoptotic Bcl-2.

Cite This Study

Julia Colston (1998) studied Acute viral myocarditis (n=40). Coxsackievirus B3 (CVB3) inoculation vs. Saline vehicle-injected controls was evaluated on Myocardial apoptosis (TUNEL-positive cells). Inoculation with coxsackievirus B3 in mice resulted in marked inflammatory activity but exceedingly rare myocyte apoptosis in the myocardium.

synapsesocial.com/papers/6a6c414e34b735385cb3ecd9 https://doi.org/10.1016/s0008-6363(97)00323-4

Topics

Myocarditis

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Expression of apoptosis-related proteins in experimental coxsackievirus myocarditis

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Topics

Expression of apoptosis-related proteins in experimental coxsackievirus myocarditis

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Topics

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