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September 1, 1997Journal of Clinical InvestigationOpen Access

Angiotensin II stimulates expression of the chemokine RANTES in rat glomerular endothelial cells. Role of the angiotensin type 2 receptor.

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Key result

Angiotensin II infusion in rats significantly stimulated glomerular RANTES expression and increased the influx of ED-1-positive macrophages/monocytes compared to solvent control (0.46 vs 0.25 cells per glomerulus, p<0.01).

Population

Cultured rat glomerular endothelial cells (GER) and male Sprague-Dawley rats (150 g body weight)

Comparison

Angiotensin II vs Solvent/vehicle control (0.9% NaCl)

Design

Preclinical, Quantitative morphologic analyses were performed in a blinded…

Follow-up

4 days (in vivo)

Authors

GWGünter WolfThe Wistar InstituteFZFuad N. ZiyadehAmerican University of Beirut Medical CenterFTFriedrich ThaissUniversität Hamburg

Discussion

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Implication

May inform renin-angiotensin blockade effects on glomerular inflammation; leaves open validation in human renal disease.

Structured PICO

P
Population
In vitro experiments using rat glomerular endothelial cells and in vivo experiments using male Sprague-Dawley rats to evaluate the effects of Angiotensin II.
I
Intervention
Angiotensin II (in vitro: 10^-8 to 10^-6 M; in vivo: 500 ng/h intraperitoneal infusion for 4 days)
C
Comparator
Solvent/vehicle control (0.9% NaCl)
O
Outcome
RANTES mRNA and protein expression, and monocyte chemotactic activitysurrogate

Main Result

Absolute Event Rate: 0.46% vs 0.25%

p-value: p=<0.01

Angiotensin II stimulates the expression of the chemokine RANTES in glomerular endothelial cells via the AT2 receptor, promoting monocyte/macrophage infiltration.

Limitations

  • Short-term infusion period (4 days) does not assess long-term structural changes such as glomerulosclerosis.
  • In vitro findings in isolated glomerular endothelial cells may not fully represent complex in vivo mechanisms.
  • Glomerular endothelial cells may not be the only source of the Angiotensin II-mediated increase in RANTES production in vivo.

Cite This Study

Wolf et al. (1997) studied Glomerular inflammation. Angiotensin II vs. 0.9% NaCl (solvent) was evaluated on ED-1-positive cells per glomerular cross-section (p=<0.01). Angiotensin II infusion in rats significantly stimulated glomerular RANTES expression and increased the influx of ED-1-positive macrophages/monocytes compared to solvent control (0.46 vs 0.25 cells per glomerulus, p<0.01).

synapsesocial.com/papers/6a6cb9fe35aa2c282cdfa69dhttps://doi.org/10.1172/jci119615
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Angiotensin II Is Mitogenic for Cultured Rat Glomerular Endothelial Cells1996 · 81 citations
  2. 2Activation of human peripheral monocytes by angiotensin II1994 · 210 citations
  3. 3The angiotensin AT2-receptor mediates inhibition of cell proliferation in coronary endothelial cells.1995 · 915 citations
  4. 4Molecular cloning of a novel angiotensin II receptor isoform involved in phosphotyrosine phosphatase inhibition.1993 · 716 citations
  5. 5Angiotensin II receptor subtypes in the kidney.1993 · 65 citations